通过使用链接蛋白FRET平台的SARS-CoV-2主蛋白酶对所有11个病毒聚蛋白分裂部位处理事件的动态比较

相关实验视频

Updated: Jun 26, 2025

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation
07:53

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation

Published on: January 9, 2019

33.2K

Calem Kenward1, Marija Vuckovic1, Mark Paetzel2

  • 1Department of Biochemistry and Molecular Biology and Centre for Blood Research, The University of British Columbia, Vancouver, British Columbia, Canada.

The Journal of biological chemistry
|May 16, 2024
PubMed
概括

SARS-CoV-2的主要蛋白酶 (Mpro) 是一种关键的药物标. 一个新的FRET系统揭示了Mpro以不同的催化效率处理病毒多蛋白,这表明nsp处理的首选顺序.

关键词:
3CL 蛋白酶是一种3CL蛋白酶.3CLpro 3CLpro是什么 3CLpro是什么 3CLpro是什么费特·弗雷特 (FRET FRET) 是一家在美国的船只.在Mpro Mpro中使用.这就是SARS-CoV-2病毒.冠状病毒冠状病毒病毒酶动力学 酶动力学主要蛋白质酶的主要蛋白质酶这是一种多蛋白质多蛋白质.蛋白质酶基质的基质病毒蛋白酶是一种病毒蛋白酶.

更多相关视频

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods10:40

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Published on: December 21, 2019

26.0K
Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation19:23

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

Published on: January 16, 2019

9.2K

相关概念视频

Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.5K
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

3.8K
The Proteasome Structure01:17

The Proteasome Structure

The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
The proteasome is an...
747
Mechanical Protein Function01:58

Mechanical Protein Function

2.0K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K