拼写错误或"混" - - 癌症研究出版物中的不可核实和未知的细胞系
Danielle J Oste1,2, Pranujan Pathmendra1, Reese A K Richardson3
1School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, New South Wales, Australia.
International journal of cancer
|May 16, 2024
概括
在miR-145研究中错误识别反应物是常见的,超过一半的论文显示出错误. 非可验证的细胞系对研究完整性和可重复性构成重大挑战.
科学领域:
- 生物技术是生物技术.
- 分子生物学分子生物学
- 遗传学研究 遗传学研究
背景情况:
- 可复制的实验室研究取决于准确的试剂识别.
- 以前的工作突出了基因研究中错误识别的核酸序列,包括对miR-145.5的研究.
- 反应物的错误识别可能会损害科学发现的有效性.
研究的目的:
- 评估最近的miR-145研究中错误识别的核酸序列和细胞系的流行情况.
- 识别和描述科学文献中使用的不可核实 (NV) 细胞系标识符.
- 调查NV细胞系的起源和状态及其对研究完整性的影响.
主要方法:
- 在最近的36篇miR-145研究论文中手动验证试剂身份.
- 从miR-145论文和外部来源分析细胞系标识符.
- 调查了420篇论文,引用了8个NV标识符,以确定它们的使用和文档.
- 检查细胞系存储库数据和短串重复 (STR) 分析信息.
主要成果:
- 分析的miR-145论文中有56%含有错误识别的核酸序列,17%含有错误识别的细胞系.
- 确定了23个独特的NV细胞系标识符,其中7个通常被引用为独立细胞系 (BGC-803,BSG-803,BSG-823,GSE-1,HGC-7901,HGC-803,MGC-823).
- 没有发现描述这7个细胞系的建立的出版物;一些细胞系在存储库中被错误索引,STR配置文件经常缺失或无法识别.
结论:
- 非可验证的细胞系对研究完整性和可重复性构成重大挑战.
- 进一步调查至关重要,以澄清NV细胞系的地位和身份.
- 确保准确的试剂识别对于可靠的实验室研究至关重要.
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