维生素D3和欧米茄3组合对病原性微生物的抗菌活性
Mohammad A A Al-Najjar1, Shaymaa B Abdulrazzaq1, Shatha Al-Shaer2
1Department of Pharmaceutical Science and Pharmaceutics, Faculty of Pharmacy, Applied Science Private University, Amman, Jordan.
Combinatorial chemistry & high throughput screening
|May 16, 2024
概括
维生素D3对抗病原体具有显著的抗微生物活性,与omega-3不同. 结合维生素D3和omega-3可以增强抑制作用,但它们与安福特素B对抗Candida albicans.
科学领域:
- 微生物学 微生物学
- 营养免疫学 营养免疫学
背景情况:
- 像维生素D3和omega-3这样的补充剂调节免疫系统和肠道微生物群.
- 这些补充剂通过影响宿主内部环境来间接控制病原体的生长.
研究的目的:
- 评估维生素D3和欧米茄-3单独和组合的直接抗菌作用.
- 评估将这些补充剂与珍塔米辛或B氨基菌素结合对病原体的影响.
- 假设潜在的抗微生物机制,并建议未来的研究方向.
主要方法:
- 维生素D3和omega-3的最小抑制度 (MIC) 通过连续稀释来确定.
- 棋盘技术被用来评估对大肠杆菌,黄金葡萄球菌, Pseudomonas aeruginosa 和Candida albicans 的综合作用.
- 组合包括单独的补充剂,彼此的补充剂,以及低度的 gentamicin 或 amphotericin B.
主要成果:
- 83.3微克/毫升的维生素D3完全消灭了所有测试的微生物.
- 与维生素D3相比,欧米茄-3的抗菌作用较小3.
- 结合维生素D3和omega-3与珍塔素,可以显著降低 (6.8-7) 对格拉姆阴性和格拉姆阳性细菌的日志.
- 当维生素D3和omega-3与安福特里辛B结合使用时,对抗Candida albicans的对抗作用被观察到.
结论:
- 维生素D3对一系列致病微生物具有强大的直接抗微生物活性.
- 维生素D3和omega-3的联合使用可以增强高度的抗微生物抑制.
- 协同或对抗性相互作用取决于特定的病原体和同时使用的抗菌剂,需要进一步的机制研究.
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