依赖RNA的RNA聚合酶通过外基RNA-sRNA介导的RNAi通路调节ascospore放电
Wenping Zeng1,2, Jing Lin2,3, Jiatao Xie2,3
1Key Laboratory of Environment Change and Resources Use in Beibu Gulf, Ministry of Education, Nanning Normal University, Nanning, China.
依赖RNA的RNA聚合酶 (RdRP) Fgrdrp1,Fgrdrp2和Fgrdrp5对于Fusarium graminearum的胞分泌和成熟至关重要. 这些RdRP调节外来小干扰RNA (ex-siRNA) 生物发生,影响真菌发育.
科学领域:
- 分子生物学分子生物学
- 植物病理学 植物病理学
- 菌类遗传学 菌类遗传学
背景情况:
- 菌头部炎,一个主要的农业疾病,依赖于ascospores感染.
- 依赖RNA的RNA聚合酶 (RdRP) 是RNA干扰途径的关键,但它们在真菌发育中的作用尚未完全理解.
- 在Fusarium graminearum中Fgrdrp5的功能仍然未知,尽管已知Fgrdrp1-4.4的作用.
研究的目的:
- 为了研究Fgrdrp5在Fusarium graminearum中的功能.
- 阐明Fgrdrp1,Fgrdrp2和Fgrdrp5在阿斯科的成熟和释放中的作用.
- 了解这些RdRP在外来小干扰RNA (ex-siRNA) 生物发生和调节中的参与.
主要方法:
- 基因删除突变分析.
- 小RNA和mRNA的深度测序.
- 对小RNA和mRNA表达数据的联合分析.
- 聚生产试验. 聚生产试验.
主要成果:
- 在Fusarium graminearum中,Fgrdrp1,Fgrdrp2和Fgrdrp5对于胞分泌和成熟至关重要.
- 这三种RdRP参与以聚醇依赖的方式产生压.
- Fgrdrp1,Fgrdrp2和Fgrdrp5在外来小干扰RNA (ex-siRNA) 生物发生和积累中具有共同的功能.
- 这些RdRP通过ex-siRNA通路负面调节涉及ascospore发展和释放的基因的表达.
结论:
- Fgrdrp1,Fgrdrp2和Fgrdrp5是Fusarium graminearum中取决于分数的ex-siRNA通路的关键组成部分.
- 这些RdRP在调节真菌发育方面发挥着重要作用,特别是作为的成熟和释放.
- 脂质/糖代谢和性发育途径的缺陷也可能导致突变菌株中观察到的骨胞发育受损.
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