在Listeria monocytogenes细胞内感染中,乙醇胺利用和细菌微分区形成的作用
Ayan Chatterjee1, Karan Gautam Kaval1, Danielle A Garsin1
1Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center, Houston, Texas, USA.
Infection and immunity
|May 16, 2024
概括
乙醇胺 (EA) 代谢基因对于Listeria monocytogenes (LMO) 在宿主细胞内复制并逃避免疫检测至关重要. 在LMO感染期间,用于EA利用的细菌微分区的形成因细胞类型而异.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 病原体与宿主之间的相互作用
背景情况:
- 乙醇胺 (EA) 影响细菌病原体的殖民化和细胞内生存.
- 在像Listeria monocytogenes (LMO) 这样的细菌中,EA利用 (eut) 基因对于EA代谢至关重要.
- 以前的研究表明,EA酶分解基因的丧失会损害LMO细胞内复制.
研究的目的:
- 进一步描述EA利用在LMO宿主细胞细胞内感染中的作用.
- 在LMO感染期间研究EA利用细菌微分区 (BMC) 的形成和局部化.
- 检查EA利用突变物对宿主细胞免疫反应的影响.
主要方法:
- 产生和分析了缺乏EA调节器 (ΔeutV) 或代谢 (ΔeutB) 基因的LMO突变.
- 感染了各种宿主细胞系 (Caco-2,RAW 264.7,THP-1) 的LMO菌株.
- 用光标记的本地EUT BMC用于体外和体内可视化.
- 在受感染的Caco-2细胞中评估炎症性细胞因子表达.
主要成果:
- ΔeutV 和 ΔeutB 突变在所有测试的细胞系中显著减少了细胞内复制.
- 在LMO感染Caco-2细胞中观察到EUT BMC形成,但在巨细胞系中没有.
- 同样地,deutB和deutV突变在Caco-2细胞中增加了炎症性细胞因子表达.
- 对于LMO复制和免疫逃避来说,EA的感知和利用很重要,但BMC的形成并不总是同时发生的.
结论:
- EA的新陈代谢和调节对于最佳的LMO细胞内生长和免疫逃避至关重要.
- EUT BMC 的形成取决于宿主细胞,并且在某些细胞类型的早期感染阶段观察到.
- 了解EA利用途径为LMO病原和潜在的治疗策略提供了洞察力.
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