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通过真菌-细菌联盟确定土壤氨酸降解

Chunling Luo^1,2, Guoqing Guan^1,2, Yeliang Dai^1,2

¹State Key Laboratory of Organic Geochemistry and Guangdong Provincial Key Laboratory of Environmental Protection and Resources Utilization, Guangdong-Hong Kong-Macao Joint Laboratory for Environmental Pollution and Control, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, China.

Applied and environmental microbiology

|May 16, 2024

概括

真菌和细菌菌株共同作用,在受污染的土壤中分解多环芳 (PAH). 这种真菌有助于细菌分解PAH,增强了土壤的整体生物修复.

科学领域:

环境微生物学环境微生物学
生物修复是一种生物修复.
土壤科学土壤科学

背景情况:

菌-细菌联盟对于有机污染物去除至关重要.
在多环芳 (PAH) 生物降解过程中生物增强的机制尚不清楚.
石油污染的土壤带来了重大的环境挑战.

研究的目的:

为了研究PAH生物降解的真菌-细菌联盟的生物增大机制.
为了阐明 *Aspergillus* sp. 的作用. 在 (PHE) 降解过程中,LJD-29 (真菌) 和*Pseudomonas* XH-1 (细菌).
了解这个联盟对受污染的土壤中的微生物社区多样性的影响.

主要方法:

稳定同位素探测 (SIP) 以追踪活性PHE降解微生物.
引入了土著真菌菌株 *Aspergillus* sp. 的存在. 在LJD-29和细菌菌株*Pseudomonas*XH-1.
代谢物分析和微生物丰富度,降解效率和酶活性之间的相关性研究.

主要成果:

这两种菌株单独增强了PHE降解,但联合效果并没有显著超过单独的细菌菌株.
SIP确定了*Pseudomonas* XH-1作为主要的PHE降解剂,而*Aspergillus* sp.则是主要的降解剂. LJD-29扮演了一个配套角色.
真菌的细胞外酶活性与降解效率相关,表明真菌促进了细菌对PHE代谢物的同化.
菌-细菌组合显著改变了PHE降解微生物的多样性.

结论:

揭示了一种新的真菌-细菌生物增生机制,其中真菌支持细菌PAH降解.
真菌和细菌之间的协同相互作用对于有效的PAH生物修复至关重要.
这项研究为PAH污染土壤的"现场"生物修复提供了宝贵的见解.

关键词:

在PHE同化过程中,PHE同化积极降解微生物的微生物.细胞外酶活性的细胞外酶.菌细菌的生物增量稳定同位素探测的探测

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