在血清刺激或线粒体退出后,UHRF2在早期G1阶段积累,以延长G1和细胞周期总长度
Xiaohong Wang1, Huarui Lu1, Grace Sprangers1
1Department of Pediatrics, Division of Blood and Marrow Transplantation, University of Minnesota, Minneapolis, MN, USA.
Cell cycle (Georgetown, Tex.)
|May 16, 2024
概括
与PHD和环指域2 (UHRF2) 一样,乌比奎丁通过控制基因表达和蛋白质稳定性来调节细胞循环节奏. 失去UHRF2会延迟细胞循环的进展,并影响循环素和CDK抑制剂水平.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 与PHD和戒指域2 (UHRF2) 类似的乌比奎丁与UHRF1同源,但缺乏其DNA甲基化功能.
- 在细胞循环进展中的UHRF2的作用及其致癌或瘤抑制功能尚未完全理解.
- UHRF2与E2F1相互作用,并受到循环E/CDK2的调节,这表明它参与细胞循环控制.
研究的目的:
- 阐明UHRF2在细胞循环调节中的精确作用.
- 研究UHRF2通过哪些机制影响细胞周期基因表达和蛋白质稳定性的机制.
- 为了确定UHRF2调制对细胞周期进展的影响.
主要方法:
- 在CRISPR/Cas9基因编辑中删除UHRF2.2.
- 药理上抑制CDK1以研究UHRF2的动态.
- 细胞周期阶段过渡时间的分析.
- 定量循环素,CDK抑制剂 (例如,p27KIP1) 和视网母细胞瘤酸化水平.
主要成果:
- 在早期G1中观察到UHRF2表达,并在M阶段丢失,由于CDK1抑制,耗尽可逆.
- 使用CRISPR/Cas9删除UHRF2显著延迟了细胞周期的所有阶段的进展.
- 失去UHRF2会导致循环素水平增加和p27KIP1升高,减少视网膜母细胞瘤酸化,并延长G1/S过境.
结论:
- UHRF2 作为细胞周期节奏的关键调节者.
- UHRF2通过复杂的细胞周期基因表达和蛋白质稳定性的调节来控制细胞周期进展.
- UHRF2复杂的相互作用和反循环突出了它在维持细胞周期动态中的核心作用.
更多相关视频
12:02Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Published on: June 6, 2017
27.3K
08:37Use of the Pyrimidine Analog, 5-Iodo-2′-Deoxyuridine IdU with Cell Cycle Markers to Establish Cell Cycle Phases in a Mass Cytometry Platform
Published on: October 22, 2021
2.9K
相关概念视频
02:37DNA Damage can Stall the Cell Cycle
9.1K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.1K
01:23Negative Regulator Molecules
35.3K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.3K
02:39Positive Regulator Molecules
5.4K
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
5.4K
01:27Molecular Factors Affecting Cell Division
3.1K
Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
Several proteins function as internal regulators to ensure each cell cycle stage is completed faithfully before proceeding to the next. Regulator molecules may act directly or influence the activity or production of other...
3.1K
02:15M-Cdk Drives Transition Into Mitosis
5.6K
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
5.6K
01:28The Cell Cycle Control System
2.8K
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
2.8K