一个NAC转录因子抑制与糖含量相关的模块,并调节耐受性
Su Li1,2, Ji Bo Yang1,3, Jia Qi Li1,2
1State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
Plant physiology
|May 16, 2024
概括
一种新型的NAC转录因子,NAC070,通过调节根细胞壁组件并与其他关键因素相互作用,增强了Arabidopsis中的耐受性. 这一发现为植物适应酸性土壤提供了新的见解.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 生物化学 生物化学
背景情况:
- (Al) 的毒性严重限制了酸性土壤中的作物生产率.
- 植物Al耐受性背后的分子机制尚未完全理解.
- 转录调节在植物对环境压力的反应中起着至关重要的作用.
研究的目的:
- 为了识别和表征涉及到阿拉比多西斯 (Arabidopsis thaliana) 中Al耐受性的转录因子.
- 阐明ANAC070在调节积和植物对毒性的反应中的作用.
- 研究由ANAC070.0.调节的分子相互作用和途径.
主要方法:
- 通过基因查和分子分析识别NAC转录因子 (ANAC070).
- 在野生类型和突变的阿拉比多普西斯根中积的分析.
- 酵母的一种混合测试用于研究蛋白质相互作用.
- 双露西法酶记者测定,RT-qPCR和GUS分析用于研究基因调节.
- 对淘汰突变体的表型分析,以评估Al敏感性.
主要成果:
- ANAC070的表达和蛋白质水平是由Al诱导的,而它的缺失会增加Al的敏感性.
- anac070突变体在根部,特别是细胞壁中呈现出更高的Al积累,细胞内含有改变的半纤维素和糖水平.
- ANAC070直接抑制了ANAC017的表达,这是一种涉及到氧黄糖修饰的基因.
- ANAC017的淘汰部分挽救了anak070突变体的Al敏感性,这表明anak070通过多个途径起作用.
- 已知的Al耐受性因子STOP1也可能参与ANAC070介导的Al耐受性.
结论:
- ANAC070是阿拉比多普西斯中Al耐受性的关键调节剂.
- ANAC070通过调节根细胞壁组成和抑制ANAC017-XTH31模块来促进Al耐受性.
- 这些发现揭示了植物对Al耐受性的新型转录性调节网络.
相关概念视频
Prokaryotic Transcriptional Activators and Repressors
21.0K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
21.0K
Operons
49.0K
Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by...
49.0K
Eukaryotic Transcription Inhibitors
9.8K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
9.8K
Transcription Attenuation in Prokaryotes
15.3K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
15.3K
Co-activators and Co-repressors
7.3K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.3K
Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K


