酸盐1-介导的酸盐从根到芽的转移调节了植物的花过渡
Senhuan Dai1, Huiying Chen1, Yutao Shi1
1College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
Journal of experimental botany
|May 16, 2024
概括
酸盐运输体PHOSPHATE1 (PHO1) 对于植物开花时间至关重要. 由于破坏酸盐运输和改变花基因表达,PHO1的损失延迟了开花,突出显示了它在将营养状况与生殖发育相结合方面的作用.
科学领域:
- 植物生物学 植物生物学
- 分子植物生理学分子植物生理学
- 营养信号传递 营养信号传递
背景情况:
- 众所周知,营养会影响花过渡,但分子机制在很大程度上仍然未知.
- 阿拉比多的酸盐输送物PHOSPHATE1 (PHO1) 对于将酸盐从根部转移到芽部至关重要.
- 之前还没有研究PHO1在调节花过渡中的作用.
研究的目的:
- 研究阿拉比多普西斯1 (PHO1) 在调节花过渡中的作用.
- 为了阐明PHO1因的可用性而影响开花时间的机制.
- 探索其他植物物种在花过渡中的PHO1功能保护.
主要方法:
- 在各种条件下分析了花期的阿拉比多普西斯塔利亚纳基因突变 (pho1,pho2,spx1,spx2).
- (Pi) 补充实验在不同的植物部位 (花,开花) 进行.
- 进行了移植试验,以评估根到芽转移的作用.
- 用基因表达分析来检查花通路基因 (例如,FT) 和信号通路 (例如,斯酸) 的调节.
- 在大米 (Oryza sativa) 中进行了同源基因分析.
主要成果:
- 在长日和短日条件下,pho1淘汰突变体表现出延迟的开花.
- 在pho1突变体中,晚期开花被部分通过在花圈或芽中补充Pi来挽救,并通过移植实验表明根到芽转移受损.
- 淘汰SPX1和SPX2部分挽救了pho1突变的晚期开花表型.
- 失去PHO1功能导致了像FT这样的花激活剂的抑制表达,并诱导了花抑制剂在芽中的表达.
- 鉴定出雅斯蒙酸信号传递是晚期开花表型的部分责任途径.
- 米类同类物PHO1;2在调节花转变方面发挥了类似的作用.
结论:
- 1 (PHO1) 在将营养与植物花期调节的整合中起着至关重要的作用.
- 由于PHO1损失而导致酸盐转位受损会影响花通路基因的表达,导致花期延迟.
- PHO1代表了一个潜在的分子标,用于操纵作物中营养介导的开花时间.
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