相关实验视频
Updated: Jun 26, 2025

14:32
Polysome Profiling in Leishmania, Human Cells and Mouse Testis
Published on: April 8, 2018
18.1K
莱什马尼亚核糖体蛋白 (RP) 相似基因相互补偿表达,保持蛋白质原生水平
Francisca S Borges1, José C Quilles1, Lucas B Lorenzon1
1Department of Cell and Molecular Biology, Ribeirão Preto Medical School, FMRP/USP-University of São Paulo, São Paulo, Brazil.
PloS one
|May 16, 2024
概括
在Leishmania寄生虫中,重复的核糖体蛋白基因表现出差异性表达,经过翻译调节. 补偿表达式表明对象之间的功能性保存,受3'未翻译区域的影响.
科学领域:
- 分子寄生虫学 分子寄生虫学
- 基因规则 基因规则
- 核糖体生物学 核糖体生物学
背景情况:
- 莱什马尼亚寄生虫具有多个核糖体蛋白 (RP) 基因的复制.
- 类似的RP基因往往具有不同的未翻译区域 (UTR),表明不同的调节机制.
- 了解RP基因表达对于寄生虫的发展和生存至关重要.
研究的目的:
- 为了研究莱什马尼亚大菌中重复的核糖体蛋白基因 (RPS16和RPL13a) 的表达特征.
- 为了确定RP等式的差异表达背后的调节机制.
- 探索RP异型的功能性保存和转录后调节.
主要方法:
- 通过CRISPR/Cas9基因组编辑,产生RPS16和RPL13a对应物的淘汰 (Δ) 和内源标记细胞系.
- 使用标记和淘汰细胞系分析差异性蛋白质表达.
- 评估转录水平和核糖体概况,以调查转录后调节.
- 在3' UTRs内识别潜在调节平行表达的cis元素.
主要成果:
- 在整个寄生虫发育过程中观察到RPS16和RPL13a异型的差异表达,其中一种异型始终更丰富.
- 补偿性蛋白质表达发生在对应物被删除时,表明功能性保存没有转录水平变化.
- 鉴定了与3' UTRs相互作用的异位基因特异性RNA结合蛋白,这表明它们在转录后调节中的作用.
- 在3' UTRs中发现了保留和排他性的cis元素,与RP异型的差异丰度相关.
结论:
- 莱什马尼亚RP类比的差异表达主要在翻译后的水平上得到调节.
- 在RP对应物之间存在功能性保护,补偿机制确保了蛋白质平衡.
- 3' UTRs 在调节莱什马尼亚RP基因对应物表达方面发挥着至关重要的作用.
相关概念视频
Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Riboswitches
8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K
Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
Regulated Protein Degradation
7.2K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
7.2K
Covalently Linked Protein Regulators
6.8K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
6.8K
Regulation of Nuclear Protein Sorting
2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K

