基于黑激素受体1A基因多态的公羊的基因定型:在 sire 选择中的一个工具?
Victoria Peña-Delgado1, Agustí Noya1, Melissa Carvajal-Serna1
1Grupo BIOFITER-IUCA, Universidad de Zaragoza, Facultad de Veterinaria, Miguel Servet 177, 50013 Zaragoza, Spain.
Reproduction, fertility, and development
|May 16, 2024
概括
黑激素受体1A基因 (MTNR1A) 中的多态性影响雄羊精液质量. 特定突变 (C>T,G>A) 可以降低精子健康,特别是在生殖季节.
科学领域:
- 动物繁殖 动物繁殖
- 遗传学 遗传学 是一个
- 生物化学 生物化学
背景情况:
- 黑激素受体1A (MTNR1A) 基因在绵羊的生殖性能中起作用.
- 在MTNR1A中的特定多态性与生殖成功的变化有关.
研究的目的:
- 研究MTNR1A基因中Rsa I和Mnl I多态性对公羊精液质量的影响.
- 评估这些多态性对全年各种精子参数的影响.
主要方法:
- 针对MTNR1A基因的Rsa I和Mnl I等位基因变异的18只Rasa Aragonesa公羊的基因定型.
- 每月对精子运动,形态,膜完整性,活性氧物种 (ROS),脂素 (PS) 转移,DNA碎片化和电容状态的射精进行分析.
- 使用混合ANOVA对精液质量的季节性和多态性影响的评估.
主要成果:
- 两种Rsa I和Mnl I多态都影响了膜完整性和具有低ROS和没有PS转位的活力精子.
- Rsa I 多态性也影响了精子运动性和DNA完整性.
- 多态性之间的相互作用表明,当携带T或A等位基均时,对精液质量产生负面影响,在繁殖季节观察到的差异更大.
结论:
- 雄羊的MTNR1A基因中的突变 (C>T在Rsa I,G>A在Mnl I) 可以降低精液质量.
- 在绵羊繁殖计划中,MTNR1A基因型定型为选择优秀雌雄提供了有价值的工具.
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