通过深溶性溶剂和溶性酸沉提取的虎粉中的蛋白质:结构,功能和营养的比较研究
Xinyu Jiang1, Feng Gao1, Yongliang Ma1
1College of Food Science and Engineering, Jilin University, Changchun 130062, PR China.
Food chemistry
|May 16, 2024
概括
这项研究优化了使用深溶解剂 (DES-TNP) 提取老虎坚果蛋白质,与传统方法相比,实现了更高的纯度和更好的功能性质. 这种新的方法提高了虎蛋白质的利用率.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物化学 生物化学
- 可持续化学 可持续化学
背景情况:
- 虎蛋白 (TNP) 具有高营养价值,但高效的提取方法对于其全面利用至关重要.
- 传统的蛋白质提取方法在效率和纯度方面可能存在局限性.
研究的目的:
- 为了优化提取虎蛋白的提取参数,使用深度欧性溶剂 (DES).
- 为了比较DES提取的TNP (DES-TNP) 的纯度,结构特征,功能性质和营养价值,与通过溶性酸沉 (ASAE-TNP) 获得的.
主要方法:
- 优化DES提取参数:键捐赠者与键接受者的比率 (HBD:HBA = 5:1),液体与固体的比率 (11:1) 和水分含量 (15%).
- 使用纯度测定,密度功能理论 (DFT),显微分析,光谱学 (二级和三级结构),分子重量分布分析和功能性质评估 (溶解性,乳化,发泡) 来描述提取的蛋白质.
- 氨基酸组成和体外消化能力的比较.
主要成果:
- 与ASAE-TNP (67.48%) 相比,优化的DES提取产生了明显更高纯度 (76.21%) 的TNP (DES-TNP).
- DFT证实了DES的合成和与TNP的相互作用.
- 与ASAE-TNP相比,DES-TNP在pH 13处表现出明显的结构特征和优越的溶解性,乳化和发泡特性.
- 这两种方法都显示了类似的氨基酸概况和消化能力,但DES-TNP的总氨基酸含量更高.
结论:
- 深度环氧化溶剂提取为获得具有增强功能性质的高纯度老虎坚果蛋白质提供了一种新且有效的方法.
- 这种优化的DES方法为食品行业全面利用老虎坚果资源提供了一个有希望的途径.
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