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通过手工克隆生产抗分裂植物酶转基因猪

Mingyuan Zhang¹, Jun Zhang², Haowen Yang³

¹Life Science Institute, Guangxi Medical University, Nanning, 530021, China; College of Animal Science and Technology, Guangxi University, Nanning, 530005, China; Laboratory Animal Center, Guangxi Medical University, Nanning, 530021, China.

|May 16, 2024

概括

生产抗分裂植物酶的转基因猪是为了减少来自动物废物的污染而制造的. 一只猪显示出显著的酶活性,改善了的消化和减少便排放.

科学领域:

动物生物技术动物生物技术
环境科学环境科学
遗传学遗传学是一个

背景情况:

强化畜牧业产生了大量的肥废物,其中含有植物酸.
植物酶可以减轻来自单胃动物便的污染.
之前的植物酶生产猪 (Enviropig) 在消化过程中出现了酶失活的问题.

研究的目的:

使用手工克隆生成抗裂裂植物酶转基因猪.
改进转基因构造以提高酶的稳定性和有效性.
评估这些猪对消化和环境污染的影响.

主要方法:

改善了对抗分裂植物酶 (Cafp) 的转基因构造.
手工克隆技术可以产生转基因猪.
聚合酶连锁反应,测序和酶活性试验用于验证.
的消化速度和便排放分析.
免疫组织化学验证组织特异性基因表达.

主要成果:

在14只克隆小猪中,有7只携带Cafp转基因.
一只活猪在四个月时表现出唾液植物酶活性 (0.155 FTU/mL).
最高的消化率达到了59.2%,使便排放量减少了25.4%.
转基因表达被证实在腺体中具有组织特异性.

结论:

通过手工克隆成功生产了抗分裂的植物酶转基因猪.
这些猪代表了管理营养和畜牧业污染的生物战略.
可能需要进一步的研究来优化所有转基因动物的酶激活.

关键词:

抗裂开的植物酶可以抵抗裂开.手工克隆的人工克隆转基因猪是转基因的猪.

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