相关实验视频

Updated: Jun 26, 2025

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

对盐土中抗生素耐药性荷尔梅斯的关键见解:盐度调节的影响

Jianbin Sun¹, Dan Zhang¹, Shuang Peng²

¹State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; University of Chinese Academy of Sciences, Beijing 100049, China; Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing 210095, China.

Journal of hazardous materials

|May 16, 2024

概括

盐度显著影响土壤抗生素耐药性基因 (ARG),中等盐度水平显示出最高的丰度和风险. 随机过程驱动ARG社区在盐土中的聚集.

科学领域:

环境微生物学环境微生物学
基因组学就是基因组学.
土壤科学土壤科学

背景情况:

土壤是抗生素耐药性基因 (ARG) 的主要储存库.
盐度对土壤抗生素耐药性的影响尚不清楚.
了解这些因素对于管理抗生素耐药性至关重要.

研究的目的:

调查不同盐度水平对土壤中ARG的发生,健康风险,驱动因素和聚集的影响.
阐明盐度,细菌相互作用,功能基因和ARG增殖之间的关系.
确定盐水环境中ARG的主要驱动因素和组装机制.

主要方法:

使用高通量PCR (qPCR) 来分析ARG.
评估了细菌群体的组成和功能基因表达.
中立社区模型被用来分析ARG社区集会.

主要成果:

总共检测到206种亚型ARG,其中中等盐度的土壤显示出ARG的丰度和数量最高,包括高风险的ARG.
细菌相互作用和上调的功能基因 (ROS产生,膜透性,ATP合成) 与中等盐度下的ARG增殖有关.
ARG的主要驱动因素是从细菌群落转移到带有盐度增加的移动遗传元素,而能源供应是极端盐度的重要因素.

结论:

盐度是影响土壤抗生素耐药性的关键因素.
随机过程控制了盐土中ARG群体的聚集.
这项研究提供了关键的见解,对ARGs的命运和传播,以应对盐度变化.

关键词:

抗生素耐药性抗生素耐药性装配过程装配过程细菌社区的细菌社区盐土是一种盐土.盐分盐分盐分盐分盐分

更多相关视频

Bioprospecting of Extremophilic Microorganisms to Address Environmental Pollution

Bioprospecting of Extremophilic Microorganisms to Address Environmental Pollution

Published on: December 30, 2021

Application of the Intelligent High-Throughput Antimicrobial Sensitivity Testing/Phage Screening System and Lar Index of Antimicrobial Resistance

Application of the Intelligent High-Throughput Antimicrobial Sensitivity Testing/Phage Screening System and Lar Index of Antimicrobial Resistance

Published on: July 21, 2023

相关概念视频

Responses to Salt Stress

Responses to Salt Stress

Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.

Antibiotic Selection

Antibiotic Selection

Surface Membrane Barriers

Surface Membrane Barriers

The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...