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来自Aspergillus niger的溶解多糖胺单氧化酶有效地促进了米的微生物发酵

Ting Li¹, Kehui OuYang¹, Qinghua Qiu¹

¹Jiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, Nanchang Jiangxi 330045, China.

Animal bioscience

|May 16, 2024

概括

阿斯伯吉路斯尼格尔溶解多糖胺单氧化酶 (AnLPMO) 增强了米的消化能力和在反动物中挥发性脂肪酸的产生. 这种酶显示出作为料添加剂的潜力,通过促进纤维料的微生物发酵来改善反动物的生产.

科学领域:

生物技术是生物技术.
动物科学动物科学
微生物学微生物学

背景情况:

米草是主要的农业副产品,具有巨大的反动物料潜力.
由于其复杂的纤维素结构的低消化能力限制了其利用.
酶预处理提供了一个有前途的策略,以提高纤维料的营养价值.

研究的目的:

研究Aspergillus niger溶解多糖胺单氧化酶 (AnLPMO) 在改善米的微生物发酵中的有效性.
分析AnLPMO对米的物理和化学特性的影响.
评估AnLPMO对反动物发酵参数和微生物群落组成的影响.

主要方法:

AnLPMO的异质表达和特征.
使用富里埃变换红外光谱学和X射线光电子光谱学对米的表面分析.
试验室转黄发酵测试使用16S高通量测序和qPCR来评估消化能力,气体产生,挥发性脂肪酸和微生物群体.

主要成果:

一个LPMO有效地化了大米草西兰和Avicel,降低了表面碳含量.
补充AnLPMO显著增加了体外干物质消化性 (IVDMD),总气体产量和挥发性脂肪酸 (VFA) 度.
一个LPMO刺激了关键纤维降解食细菌的生长,提高了整体发酵效率.

结论:

一个LPMO显示出显著的潜力,以改善食动物的米的营养价值和消化能力.
该酶能够修改米的结构并促进有益的微生物生长的能力支持其作为料添加剂的应用.
进一步的研究可以探索AnLPMO在体内应用,以优化反动物生产系统.

关键词:

试管体内乳液发酵乳液发酵溶解聚糖单氧化酶的溶解方法微生物社区微生物社区在米的草.表面结构表面结构

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