输血相关细菌基准菌株的特征化,在冷解形式
Marcel Prax1, Carl P McDonald2, Isabelle Bekeredjian-Ding1
1Paul-Ehrlich-Institut, Langen, Germany.
Vox sanguinis
|May 16, 2024
概括
淋化输血相关细菌参考菌株 (TRBRS) 为血液安全研究提供了一个用户友好的格式. 这种新格式确保了稳定性和可行性,改善了输血安全的质量控制.
科学领域:
- 微生物学 微生物学
- 输血医学 输血医学
- 食品和药物安全 食品和药物安全
背景情况:
- 目前的输血相关细菌参考菌株 (TRBRS) 需要超冷储存和手工制备,这限制了它们的实际应用.
- 在ISBT输血传染性传染病工作组的细菌子组启动了一项研究,以开发一个更易于使用的TRBRS格式.
研究的目的:
- 验证冷化输血相关细菌参考菌株 (TRBRS),以提高血液安全评估的可用性.
- 在实验室间的研究中评估冷化TRBRS的稳定性,可行性和强度.
主要方法:
- 两种细菌菌株,Klebsiella pneumoniae和Staphylococcus aureus,均以冷的形式制造.
- 11个国际实验室评估了冷化细菌在血小板缩物 (PC) 中的清单,识别和生长动力学.
主要成果:
- 与传统格式相比,冷化不会影响细菌菌株的生长特性.
- 解冷的TRBRS允许直接,少量的 (大约. 30细菌) 在PC中进行输血相关实验.
- 解冷式形式在不同温度下表现出长期稳定性,并在PC中直接再水化后保持生命力,在实验室间测试中100%的增PC显示出细菌生长.
结论:
- 化TRBRS为输血相关研究提供了用户友好和稳定的材料.
- 预计冷化TRBRS的改进特性将增加其在输血安全措施的质量控制中的使用.
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