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相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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相关实验视频

Updated: Jun 26, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
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无机械扫描的多色超分辨率成像,采用衍射点阵照明.

Ning Xu1, Sarah E Bohndiek2,3, Zexing Li4

  • 1State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing, 100084, China.

Nature communications
|May 16, 2024
PubMed
概括

我们开发了无机械扫描的多色超高分辨率显微镜 (MCoSM),用于高效的多色超高分辨率成像. 这种新方法为纳米研究提供可调节的分辨率和高质量的照明.

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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
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Conducting Multiple Imaging Modes with One Fluorescence Microscope
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相关实验视频

Last Updated: Jun 26, 2025

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High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
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科学领域:

  • 光学显微镜是一种光学显微镜.
  • 纳米技术纳米技术
  • 生物物理学的生物物理.

背景情况:

  • 点扫描显微镜正在推进超高分辨率成像.
  • 现有的多焦技术可以实现高速成像,但缺乏高质量照明的高效多色功能.

研究的目的:

  • 引入没有机械扫描的多色超分辨率显微镜 (MCoSM).
  • 为了实现无机械扫描的超高分辨率成像,具有可调节的分辨率和视野.
  • 为分子相互作用的纳米尺度成像提供一个多功能平台.

主要方法:

  • 使用空间光调节器进行衍射点阵照明.
  • 实施了具有不同颜色照明和相位移扫描的时间共享过程.
  • 通过不同的照明参数 (100-2,500秒) 证明可调节的分辨率和视野.

主要成果:

  • 成功实现了无机械扫描超分辨率成像.
  • 展示了可调节的分辨率和有效的视野.
  • 进行了光珠的高分辨率四色成像.

结论:

  • MCoSM为多色超高分辨率成像提供了一种高效的解决方案.
  • 该技术扩展了光谱成像能力,同时保持了空间灵活性.
  • MCoSM是复杂生物样本纳米尺度研究的一个有前途的平台.