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一个完全分类型解析的,几乎没有缺口的小麦条纹生真菌基因组组合

Jierong Wang^1,2,3, Yiwen Xu¹, Yuxi Peng¹

¹College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, 712100, China.

Scientific data

|May 16, 2024

概括

这是破坏性小麦条纹菌,Puccinia striiformis f. sp. 的第一个完整的基因组组. 三子 (Pst),是生成的. 这种高质量的组件为理解Pst提供了基础.

科学领域:

植物病理学植物病理学
基因组学就是基因组学.
菌类遗传学菌类遗传学

背景情况:

条纹生,是由Puccinia striiformis f. sp. 引起的三 (Pst),是对小麦生产的重大全球威胁.
Pst表现出一个复杂的宏环-异构生命周期与二核生物的 urediniospores.

研究的目的:

为了产生第一个完全分类型解决的,染色体尺度的基因组组合的Pst.
为研究Pst遗传变异和进化建立一个基础资源.

主要方法:

利用PacBio HiFi测序技术进行高精度的长时间读取.
采用三重组合策略来解决和组装哈普罗型.
对生成的基因组组件进行了严格的质量评估.

主要成果:

在两种Pst单元型 (75.59 Mb和75.91 Mb) 中实现了几乎没有差距的染色体尺度组合.
组件由18个伪染色体组成,具有高密度N50值 (4.17 Mb和4.60 Mb).
预测了15046和15050个蛋白质编码基因,BUSCO分数高 (97.7%和97.9%).

结论:

高质量的Pst基因组组装是真菌基因组学的一个重大进步.
这一资源将促进未来对Pst遗传多样性,毒性和进化轨迹的研究.
能够更深入地了解小麦与的相互作用,并开发出改进的疾病管理策略.

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.