在不同的氧气条件下,通过空气化固态培养生产的基于森林垃圾的生物肥料的化学性质和微生物群落组成的变化
Lucie Miché1, Alizée Dries1, Inès Ben Ammar1
1IMBE, Aix Marseille Univ, Avignon Univ, CNRS, Marseille, IRD, France.
概括
发酵森林垃圾 (FFL) 生物肥料生产需要严格的无氧条件. 引入氧气会改变微生物群落,产生不良结果和潜在的有害菌.
科学领域:
- 农业微生物学 农业微生物学
- 生物技术是生物技术.
- 土壤科学 土壤科学
背景情况:
- 发酵森林垃圾 (FFL) 是东亚和拉丁美洲的传统生物肥料,通过对森林垃圾与农业副产品的无氧发酵来生产.
- 工艺生产方法依赖于农民主导的低技术工艺,需要对潜在的微生物和生物化学转化进行科学理解.
研究的目的:
- 从化学和微生物学上描述发酵森林垃圾 (FFL) 生产过程及其最终产品.
- 研究氧气暴露对FFL固态培养,微生物群落和生物化学输出的影响.
主要方法:
- 气体和液体色谱被用来分析挥发性有机化合物 (VOC) 和有机酸.
- 使用元编码分析来识别占主导地位的微生物群落 (细菌和酵母).
- 有控制的实验评估了无氧和受氧影响的条件对发酵的影响.
主要成果:
- 在无氧条件下,FFL发酵产生了大量的乳酸和酸,导致最终pH值低 (4.72),其中主要的微生物群体包括乳酸细菌和酵母.
- 确定了诸如乙醇和乙酸乙烯之类的关键VOCs,与二氧化碳生产率相关.
- 氧气暴露导致最终pH值高 (高达8.5),乳酸和酸不存在,并转向蛋白质细菌和潜在的致病菌 (例如,Fusarium).
结论:
- 严格遵守无毒条件对于FFL的成功产生至关重要,确保有益的微生物活性和酸化.
- 在FFL发酵过程中氧气污染可能会破坏所需的微生物生态,抑制酸性生产,并促进不良或潜在的致病微生物的生长.
- 了解微生物动力学和生物化学途径对于优化FFL生物肥料的质量和安全至关重要.
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