潜在有害单元型的种群动态:一个血统分析
Katherine D Arias1, Iván Fernández1, Juan Pablo Gutiérrez2
1Área de Genética y Reproducción Animal, SERIDA-Deva, Camino de Rioseco 1225, Gijón, 33394, Spain.
BMC genomics
|May 16, 2024
概括
潜在有害的候选基因基因可能是由SNP调用错误引起的,而不是突变或重组. 只有在血统中完全分离的等位基因才应该被认为是真正有害的,这会影响近亲繁殖的抑郁.
科学领域:
- 动物遗传学动物遗传学
- 人口遗传学 人口遗传学
- 保护基因组学 保护基因组学
背景情况:
- 低频率的单元类型可以表明与亲生繁殖抑郁症相关的潜在有害基因.
- 识别和研究这些候选单基因的动态是至关重要的,但具有挑战性.
- 为了研究等位基因动态,分析了临灭绝的戈丘阿斯图尔切尔塔猪品种的血统.
研究的目的:
- 为了识别和描述猪品种血统中的候选基因.
- 研究这些候选基因的起源和遗传模式.
- 确定候选等位基因作为潜在有害遗传变异指标的可靠性.
主要方法:
- 使用Axiom PigHDv1 Array (658,692个SNP) 来对51个家庭的471头猪进行基因型鉴定.
- 分析了4470个连接块 (LB) 和16981个不同群体和整个群体 (WP) 的等位基因.
- 亲属关系和分离分析以识别不一致性和de novo等位基因发生.
主要成果:
- 超过5,466 (32%) 的单元类型被确定为候选单元,其中73%只发生一次.
- 亲属关系分析揭示了67,742个亲子不相容性,与98.2%的候选单基因相关.
- 大多数候选等位基因都出现了de novo,表明非遗传起源,只有17个被证实是分离的.
结论:
- 候选基因基因主要是由SNP调用错误引起的基因基因掉入事件引起的,而不是突变或重组.
- 在父母/祖父母中存在候选等位基因并不证实它们的有效性;分离分析是关键.
- 只有完全分离的候选等位基因应被考虑用于评估近亲繁殖抑郁症,已确定16个候选基因.
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