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在Snf1/Mig1网络中探索碳源相关的局部化和酸化,使用基于种群和单细胞的方法

Svenja Braam¹, Farida Tripodi², Linnea Österberg^1,3,4,5

¹Department of Mathematical Sciences, Chalmers University of Technology, University of Gothenburg Sweden.

Microbial cell (Graz, Austria)

|May 17, 2024

概括

Snf1通路调节细胞能量平衡. 它的局部化,而不仅仅是酸化,是控制酵母中的葡萄糖代谢的关键,揭示了复杂的调节机制.

科学领域:

细胞生物学细胞生物学
分子和系统生物学分子和系统生物学
代谢调节代谢调节代谢调节

背景情况:

AMP激活蛋白激酶 (AMPK) 和SNF1通路对于维护真核细胞中的能量稳态至关重要.
在Saccharomyces cerevisiae (酵母菌) 中,在葡萄糖耗尽时通过酸化激活Snf1激酶是必不可少的,但不足以缓解葡萄糖抑制.
这表明额外的依赖葡萄糖的调节机制控制了该途径的功能.

研究的目的:

研究Snf1激酶的空间动态及其与Mig1抑制器的关系.
阐明Snf1局部化的作用,以应对不同度的黑色素糖和碳来源.
了解Snf1通路在细胞能量平衡中的复杂调节.

主要方法:

使用光漂白后光恢复 (FRAP) 来分析Snf1蛋白质动态.
采用非线性混合效应建模来量化Snf1定位的动力参数.
研究了Snf1酸化,Mig1定位和糖可用性之间的相互作用.

主要成果:

Snf1的失活会影响其目标Mig1.1的核定位.
Snf1核进口的动力学是由非发酵碳来源的存在调节的.
证明了Snf1局部化动态与其酸化状态之间的相关性.

结论:

Snf1局部化是葡萄糖去抑制过程中的关键调节层,补充其酸化状态.
这项研究为Snf1通路的复杂调节提供了新的见解,Snf1通路对于细胞适应环境线索至关重要.
这些发现促进了对葡萄糖稳态的理解,并突出了Snf1空间动态在调节下游目标中的重要性.

关键词:

在Snf1/Mig1网络中.在光漂白后的光恢复.非线性混合效应模型核定位的核定位营养信号的营养信号.酵母酵母是一种酵母.

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