通过NIR-II光成像技术使用ICG和BDA-ICG实时监测外围神经功能
Xiaoqi Yang1,2, Yuanyi Wang3, Chunrong Qu2
1Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, Zhengzhou, 450052, Henan, China.
Materials today. Bio
|May 17, 2024
概括
这项研究引入了印氨酸绿 (ICG) 作为一种新型的神经标志物,用于使用近红外II (NIR-II) 成像在外围神经中可视化轴质细胞运输. 一种新的追踪仪,BDA-ICG,提供了更好的稳定性和神经损伤的成像.
科学领域:
- 神经科学是一个神经科学.
- 生物医学成像技术 生物医学成像技术
- 分子生物学分子生物学
背景情况:
- 对于神经解剖学的传统光追踪器在分辨率,组织透和长期动态监测轴质体运输方面存在局限性.
- 研究神经系统的复杂相互连接需要神经通路的先进成像技术.
研究的目的:
- 探索印氨酸绿 (ICG) 作为一种新型的神经标记物,用于轴质体运输.
- 开发和描述一种新的双向神经追踪器,用于增强外围神经成像.
- 为了证明ICG和BDA-ICG在可视化神经功能和损伤方面的实用性.
主要方法:
- 通过近红外II (NIR-II) 技术,研究了用于轴质体运输和外围神经成像的氨酸绿色 (ICG).
- 合成和表征了一种新的双向神经标记物,生物化德克斯氨酸-氨酸绿色 (BDA-ICG).
- 评估了BDA-ICG在体内表现,包括稳定性,神经与背景的比率,以及受伤的外周神经的成像.
主要成果:
- 通过NIR-II成像,证明ICG可以用于通过NIR-II成像检测外围神经内的运输功能.
- 与体内ICG相比,BDA-ICG表现出优越的长期稳定性和更高的神经与背景比率.
- 在24小时内使用BDA-ICG.成功地将受伤的外围神经与健康的神经区分开来.
结论:
- ICG是一种可行的神经追踪器,用于轴体运输和外围神经成像,使用NIR-II技术.
- BDA-ICG代表了神经追踪器的一个有希望的进步,为长期监测和伤害检测提供了改进的特性.
- 临床上可用的具有NIR-II发射特性的染料,如ICG,对神经成像应用具有重大潜力.
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