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人类血衍生外体:主动和被动药物加载方法对药物输送的影响
Rabia Gul1, Hamid Bashir2, Muhammad Sarfraz3
1Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, 22060 Abbottabad, Pakistan.
概括
人类等离子体衍生的外体组显示出作为药物载体的前景. 与超声波相比,冷解方法提供了优越的甲状腺酸荷载,并保持了外体完整性,这表明它有可能成为有效的药物递送系统.
科学领域:
- 生物技术是生物技术.
- 纳米医学是一种纳米医学.
- 药理学 药理学是指药理学的学科.
背景情况:
- 外体是来自细胞的纳米级囊泡,越来越多地被认为具有治疗潜力.
- 有效地将治疗剂加载到外体中,对于它们在药物输送中的应用至关重要.
- 人类等离子体衍生外体为纳米载体开发提供了一个易于获得的来源.
研究的目的:
- 通过使用主动和被动加载技术,评估人体血衍生的外体作为药物载体.
- 为了比较不同加载方法的有效性,加载效率和膜稳定性.
- 为了研究甲基酸盐 (MTX) 封装在外体内.
主要方法:
- 从人体血中通过微分离心和超分离分离分离分离和超.
- 使用超声波和冷解方法将甲基 (MTX) 装入外体.
- 外体体的表征包括粒子大小分析,传输电子显微镜和药物载荷能力评估.
主要成果:
- 人类等离子体衍生外体体的平均尺寸为162.15nm,泽塔电位为-30.6mV.
- 结解方法实现了较高的MTX封装 (64.5%) 与超声波 (55.5%) 相比.
- 结解负荷保持了外体完整性和细胞活力,比超声波更好,这导致了膜破坏.
结论:
- 结解方法是一种优质的技术,用于将甲状腺素加载到人体血衍生的外体.
- 通过冷解装载的等离子体衍生外体具有作为药物输送载体的巨大潜力.
- 这种方法确保了药物加载的改善,并为治疗应用保护了外体的结构完整性.
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