通过增强抗氧化剂系统,黄素对氧化压力介导的细胞损伤的保护作用
Pincha Devage Sameera Madushan Fernando1, Dong Ok Ko1, Mei Jing Piao1
1Department of Biochemistry, College of Medicine, and Jeju Research Center for Natural Medicine, Jeju National University, Jeju 63243, Republic of Korea.
Molecular medicine reports
|May 17, 2024
概括
素通过减少活性氧物种 (ROS) 和防止细胞损伤,保护肺纤维细胞免受氧化应激. 这种天然化合物增强了抗氧化防御,并抑制了细胞亡,对过氧化引起的伤害产生细胞保护作用.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 生理压力,包括过度的活性氧物种 (ROS) 生产,可以将正常纤维细胞转化为与癌症相关的纤维细胞.
- 与癌症相关的纤维细胞在各种癌症的进展中发挥着重要作用,包括胰腺癌,乳腺癌,肝癌和肺癌.
研究的目的:
- 为了研究白素在肺纤维细胞中对过氧化 (H2O2) 诱导的氧化应激的细胞保护作用.
- 阐明黄素抗氧化和抗亡作用背后的机制.
主要方法:
- 细胞活力,细胞灭绝试验 (G1亚种群,核染色),脂质过氧化和彗星试验被用来评估H2O2诱导的损伤.
- 西方斑点分析评估了与亡相关的蛋白质表达.
- 进行ROS检测 (H2DCFDA,电子自旋共振) 和抗氧化酶活性试验以评估抗氧化作用.
主要成果:
- 素预处理显著降低了细胞内ROS水平,并保护了细胞组件 (脂质,DNA) 免受H2O2诱导的损伤.
- 素增强了细胞活力,通过调节酶和Bcl-2/Bax蛋白水平来抑制细胞亡,并恢复了谷氨水平.
- 氨酸提高了关键抗氧化酶的活性和表达的调节,包括超氧化脱酶,催化酶,谷氨酸过氧化酶和血红素酶-1.
结论:
- 氨酸在肺纤维细胞中对H2O2诱导的氧化应激具有显著的细胞保护作用.
- 保护机制包括ROS清理,抑制细胞灭绝和增强内源抗氧化剂防御系统.
- 丁醇提高抗氧化酶的调节能力表明它作为治疗氧化压力相关肺部疾病的治疗剂的潜力.
相关概念视频
01:26Aging
49
Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
49
01:24Peroxisomes
12.1K
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
12.1K
01:46Electron Transport Chain: Complex I and II
13.1K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
13.1K