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在LncRNA微阵列分析中,鉴定出新型的循环lncRNAs在hidradenitis suppurativa中

Bruna De Felice¹, Pasquale De Luca², Concetta Montanino¹

¹Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania Luigi Vanvitelli, I-81100 Caserta, Italy.

Molecular medicine reports

|May 17, 2024

概括

长非编码RNAs (lncRNAs) 在皮肤健康和疾病中发挥作用. 三种特定的lncRNAs (lncRNA-TINCR,IncRNA-RBM5-ASI1和IncRNA-MRPL23-AS1) 在腹炎 (HS) 中过度表达,提供潜在的诊断和治疗点.

科学领域:

分子生物学分子生物学
皮肤病学皮肤病学
遗传学是一个遗传学.

背景情况:

长非编码RNAs (lncRNAs) 涉及各种生理和病理过程,包括皮肤疾病.
补腺炎 (HS) 或逆是一种慢性炎症性皮肤疾病,影响全球大约1%的人口,部分理解的致病因涉及免疫失调.

研究的目的:

调查 lncRNAs 在 hidradenitis suppurativa (HS) 病变发生过程中的生物相关性.
确定潜在的lncRNA生物标记物和HS的治疗点.

主要方法:

在HS患者中对lncRNAs和信使RNAs (mRNAs) 的差异表达分析.
构建一个 lncRNA-microRNA 调控网络.
使用逆转录定量PCR (RT-qPCR) 验证lncRNA表达.

主要成果:

与健康对照人相比,三种lncRNA表达特征,特别是IncRNA-TINCR,IncRNA-RBM5-ASI1和IncRNA-MRPL23-AS1,在HS患者中显著过度表达.
这些lncRNAs涉及到HS病变的调节网络.

结论:

鉴定到的 lncRNAs (lncRNA-TINCR, lncRNA-RBM5-ASI1 和 lncRNA-MRPL23-AS1) 显示出它们作为 HS 的预后预测剂的潜力.
这些发现有助于了解HS的致病机制,并可能为该疾病提供新的治疗点.

关键词:

在RT-qPCR中使用.基因调节基因调节基因调节性炎是一种补充性性炎.长非编码RNAs的编码.微RNAs 是一个微型RNA.

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相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...