牙周病原体Aggregatibacter actinomycetemcomitans JP2与大肠白细胞减少和小鼠肠道微生物组失衡相关
André L A da Costa1,2, Mariana A Soares3, Talita G B Lourenço1
1Oral Microbiology Laboratory, Institute of Microbiology Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Journal of periodontal research
|May 17, 2024
概括
口腔病原体Aggregatibacter actinomycetemcomitans (Aa) 改变肠道微生物群,并减少小鼠的免疫细胞. 这种口腔肠轴的干扰显示了病原生物的增加和开始的减少,没有显著的肠道炎症.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 口腔病原体可能会转移到肠道,可能会通过口腔-肠轴引起肠道失调.
- 高度白血病毒性的Aggregatibacter actinomycetemcomitans (Aa) 菌株与牙周病有关.
研究的目的:
- 为了研究高度白血毒性Aa菌株 (JP2) 对肠道微生物群,肠道粘膜完整性和健康小鼠免疫系统的影响.
- 评估口腔病原体转移对肠道生态系统的影响.
主要方法:
- 八周大的雄性C57BL6小鼠在4周内接受了Aa JP2或PBS的输入.
- 分析包括结肠膜本身,便物质,血清,牙组织和下.
- 评估的参数:白细胞种群,炎症媒介,粘膜完整性,膜骨损失和肠道微生物群.
主要成果:
- Aa JP2感染减少了肠道微生物的丰富性和结肠髓状细胞 (巨细胞,中性粒细胞,单细胞).
- 在受感染的小鼠中观察到Clostridiaceae,Lactobacillus taiwanensis和Helicobacter rodentium的丰富性增加.
- 受感染的小鼠表现出更高的结肠IL-6表达,脏MPO活性和轻微的膜骨损失.
结论:
- 胃内 Aa JP2 的使用会改变健康小鼠的肠道生态系统.
- 变化包括减少了髓状细胞,富含病原生物的丰富,并减少了共生动物.
- 没有观察到粘膜屏障或结肠炎症的显著破坏.
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