解读两种Pichia酵母中的多糖和它们与葡萄酒果实的分子相互作用
Cailin Kong1, Qi Zhang1, Yiqing Wang1
1College of Enology, Northwest A&F University, Yangling 712100, China.
Journal of agricultural and food chemistry
|May 17, 2024
概括
这项研究描述了来自Pichia fermentans和Pichia kluyveri的酵母多糖 (YPs),揭示了它们的结构和与葡萄酒果的相互作用. 曼诺蛋白与雌激素发生自发相互作用,影响葡萄酒.
科学领域:
- 葡萄酒学和葡萄酒化学
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- 酵母多糖 (YPs) 在葡萄酒的感官特性中起作用.
- 了解YP的结构和与风味化合物的相互作用对于葡萄酒的质量至关重要.
研究的目的:
- 来自Pichia fermentans和Pichia kluyveri的酵母多糖的特征.
- 调查年轻人计划的结构性属性.
- 分析YP和葡萄酒果之间的相互作用.
主要方法:
- 在优化条件下 (温度,pH,酶比率) 酵素提取YP.
- 使用单糖化物分析,FTIR和甲基化分析对YP分量的分离和表征.
- 异热定位热量计 (ITC) 用于研究YP-相互作用.
主要成果:
- 通过酶反应获得最佳的YP产量.
- 隔离了四个YP分量;两个被确定为葡萄糖,两个为曼诺蛋白 (YPPF-W和YPPK-W).
- 曼诺蛋白 (YPPK-W/YPPF-W) 与水果性 Ester 呈现自发相互作用,根据 Ester 特性和温度 (最佳温度为 30°C) 变化.
结论:
- 酵母多糖,特别是曼诺蛋白,与葡萄酒中的果相互作用.
- 这些相互作用受到分子量和疏水性的影响.
- 需要进一步的研究,以充分阐明YP对葡萄酒感官特征的影响.
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