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单链核酸跨膜分子载体基于正电荷螺旋式折叠体

Yunpeng Ge^1,2, Wencan Li^1,2, Jun Tian^1,2

¹State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun, 130012, P. R. China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|May 17, 2024

概括

研究人员开发了新型折叠体载体,以高效地通过膜传递单链DNA (ss-DNA). 这些带正电荷的分子显示出核酸运输和治疗应用的潜力.

科学领域:

生物化学生化学
材料科学材料科学材料科学
分子生物学分子生物学

背景情况:

核酸的跨膜传递对细胞过程和药物开发至关重要.
为了有效的核酸运输,需要先进的药物输送系统.
由于其结构的多功能性,折叠结构具有作为新型载体的潜力.

研究的目的:

开发和描述单链核酸 (ss-DNA) 的分子级跨膜载体.
调查电荷排列在折叠分子-ss-DNA相互作用和运输中的作用.
评估这些折叠体在治疗应用中的潜力,包括RNA输送.

主要方法:

合成和描述具有明显电荷对齐的Huc折叠体 (AOrnQ3Q3) 8和 (mQ3Q2) 8.
使用静电相互作用评估单链DNA (ss-DNA) 和双链DNA (ds-DNA) 的折叠分子亲和力和结合选择性.
评估SS-DNA跨脂质膜的跨膜传输效率.
使用折叠体载体进行增强EGFP-mRNA转染的演示.

主要成果:

合成了两个折叠体 (AOrnQ3Q3) 8和 (mQ3Q2) 8 ,分别具有线性和螺旋性的正电荷.
两种折叠分子都表现出强烈的DNA亲和力,并区分ss-DNA和ds-DNA,突出显示了电荷排列的重要性.
通过脂质膜实现了SS-DNA的高效传输,线性充电的折叠器显示了更高的活性.
在EGFP-mRNA转染实验中的增强表达证实了RNA传递的潜力.

结论:

引入了用于SS-DNA跨膜运输的基于折叠体的新型正电荷分子载体.
折叠体上的电荷的精确排列显著影响了它们的DNA结合和运输能力.
这些折叠聚合物对核酸跨膜运输和治疗应用具有显著的前景,特别是在RNA输送中.

关键词:

配送RNA配送RNA的时间静电相互作用静电相互作用螺旋式折叠机螺旋式折叠机分子载体是分子载体.通过膜传输的运输.

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