酸通过调节氧化应激和改善线粒体功能来改善猪部分遗传胚胎的发育
He-Xuan Qu1, Yan-Qiu Wang1, Yan-Wei Dong1
1Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China.
Reproduction in domestic animals = Zuchthygiene
|May 17, 2024
概括
酸 (CGA) 通过减少氧化应激和改善在体外培养过程中的线粒体功能来增强猪的早期胚胎发育. 这促进了更好的胚胎细胞形成和质量在 partenogenetic 激活胚胎.
科学领域:
- 生殖生物学 生殖生物学
- 生物化学 生物化学
- 胚胎学 胚胎学
背景情况:
- 酸 (CGA) 是一种强大的性抗氧化剂.
- 代激活 (PA) 用于创建早期阶段的胚胎.
- 在体外培养 (IVC) 过程中提高胚胎质量对于繁殖成功至关重要.
研究的目的:
- 调查CGA在IVC期间对猪PA早期胚胎的保护作用.
- 阐明CGA有益作用背后的机制,重点关注氧化应激和线粒体功能.
- 为了确定CGA是否影响这些胚胎中的胚胎基因组激活 (ZGA).
主要方法:
- 在IVC期间用50μM CGA对猪PA早期胚胎的治疗.
- 评估裂变和胚胎细胞形成率以及胚胎细胞质量.
- 与ZGA相关的基因表达的分析.
- 测量反应性氧物种 (ROS),谷氨,抗氧化酶活动 (甲酸酶,超氧化脱酶),线粒体膜潜力和ATP水平.
- 评估与线粒体平衡相关的基因表达 (NRF-1).
主要成果:
- CGA治疗显著增加了裂变和胚胎细胞形成率.
- 在猪PA胚胎中,CGA改善了芽细胞质量,并促进了ZGA.
- CGA降低了ROS水平,增加了谷氨丰度.
- CGA增强了catalase和超氧化物脱酶活动.
- 通过增加线粒体膜潜力和ATP水平,以及上调NRF-1的调节,CGA改善了线粒体功能.
结论:
- 在IVC期间的CGA治疗对猪PA早期胚胎产生了积极的影响.
- CGA通过减轻氧化应激和增强线粒体功能来发挥其保护作用.
- CGA具有改善猪辅助生殖技术的潜力.
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