基于图表的Actinidia chinensis的泛基因组揭示了调解水果变质的结构变异
Yingzhen Wang1,2, Pengwei Li1, Yanyan Zhu1
1Anhui Province Key Laboratory of Horticultural Crop Quality Biology, School of Horticulture, Anhui Agricultural University, Hefei, 230036, China.
概括
果 (Kiwi) 是一种果.
科学领域:
- 植物基因组学 植物基因组学
- 果物生物学 果物生物学
- 分子遗传学 分子遗传学
背景情况:
- 大多数水果在成熟过程中会失去叶绿素 (degeening).
- 基维果是独一无二的,在成熟后保留了叶绿素和绿色的肉.
- 影响果脱绿的遗传因素尚不清楚.
研究的目的:
- 为了构建基于图形的Actinidia chinensis的泛基组.
- 识别与水果颜色相关的遗传变异,特别是结构变异 (SVs).
- 为了阐明绿色肉基维果积叶绿素背后的遗传机制.
主要方法:
- 开发了一个基于图形的泛基因组,使用来自七种果品种的14个哈普洛型解析基因组组.
- 确定了非冗余基因家族和结构变异 (SV).
- 综合基因组序列和变异数据,以精确确定影响水果颜色的SV.
主要成果:
- 果泛基因组包括49,770个基因家族和84,591个SV.
- 在AcBCM基因的促销者中发现了一种新的SV.
- 这种SV导致高的AcBCM表达,通过调节AcSGR2.2,促进叶绿素积累.
结论:
- 一个高质量的果基因组揭示了广泛的遗传变异.
- 在AcBCM中发现了一种新型的SV,它是叶绿素积累和水果颜色的关键调节者.
- 这一发现为子的育种和了解水果的质量特征提供了宝贵的见解.
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