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相关概念视频

Reporter Genes02:11

Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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DNA Microarrays02:34

DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Real Time RT-PCR02:57

Real Time RT-PCR

57.2K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
57.2K

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相关实验视频

Updated: Jun 26, 2025

Massively Parallel Reporter Assays in Cultured Mammalian Cells
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Massively Parallel Reporter Assays in Cultured Mammalian Cells

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定量RUBY记者试验用于基因调节分析.

Jia Liu1, Hao Li1, Conghao Hong1

  • 1National Engineering Research Center of Tree Breeding and Ecological Restoration, State Key Laboratory of Efficient Production of Forest Resources, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.

Plant, cell & environment
|May 17, 2024
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种新的方法来使用betalain量化RUBY记者基因表达. 这种技术使基因调节的成本效益分析成为可能,有助于理解复杂的生物过程.

关键词:
这是支付宝.提取 提取 提取 提取调节基因表达 调节基因表达灯光 灯光 灯光 灯光 灯光光合作用 光合作用.活动主办人 活动主办人量化量化量化的量化.

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High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
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High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

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科学领域:

  • 分子生物学分子生物学
  • 植物科学 植物科学

背景情况:

  • 记者基因对于研究基因表达和调节至关重要.
  • RUBY 记者基因提供了可见的,非侵入性的优势,但缺乏定量分析方法.

研究的目的:

  • 为RUBY记者基因开发一种新的贝他林量化方法.
  • 将这种方法应用于研究烟草短暂表达系统中的基因调节.

主要方法:

  • 从烟草叶中提取和净化贝塔林.
  • 定量测量贝塔林度. 贝塔林度的定量测量.
  • 使用烟草的短暂表达系统.

主要成果:

  • 通过使用 betalain 成功量化了 RUBY 基因表达水平.
  • 通过转录因子CPD25和CPD45.5研究了ARC5的转录调节.
  • 通过G-box和I-box元件通过HY5和PIF通过光介导调节RCA和Rbcs1A.

结论:

  • 贝他林量化方法为研究基因表达调节机制提供了具有成本效益和直观的策略.
  • 这种方法提高了RUBY记者基因对定量分析的实用性.