米SRO1a有助于Xanthomonas TAL效应因子介导的宿主敏感基因表达
Satomi Yoshimura1, Ayaka Yoshihisa1, Yusei Okamoto1
1Department of Advanced Bioscience, Graduate School of Agriculture, Kindai University, Nakamachi, Nara 631-8505, Japan.
Plant & cell physiology
|May 17, 2024
概括
植物病原体称为Xanthomonas使用转录激活器类 (TAL) 效应器引起疾病. 这项研究表明,与RCD ONE (SRO) 类似的米蛋白,OsSRO1a和OsSRO1b,增强了TAL效应因子诱导的基因转录,影响了作物易感性和防御能力.
科学领域:
- 植物病理学 植物病理学
- 分子植物-微生物相互作用
- 植物分子生物学 植物分子生物学
背景情况:
- 桑托莫纳斯物种是重要的作物病原体,导致大量的收获损失.
- 转录激活器样 (TAL) 效应因子是Xanthomonas分泌的毒性因子,可以操纵宿主基因表达.
- 精确的分子机制,TAL效应器激活宿主基因转录并没有完全理解.
研究的目的:
- 阐明大米类似于RCD ONE (SRO) 家族蛋白质在TAL效应器介导转录中的作用.
- 研究植物核中的TAL效应器和OsSRO蛋白之间的相互作用.
- 确定OSSRO蛋白对宿主易感性和防御基因表达的影响.
主要方法:
- 酵母双混合或共同免疫沉试验检测蛋白质与蛋白质相互作用.
- 在大米原塑中进行交换活化测试,以测量促进体活性.
- 在野生型和 ossro1a 突变大米植物中的基因表达的分析.
- 定量实时PCR (qRT-PCR) 来评估与防御有关的基因的表达.
主要成果:
- TAL效应体被证明与植物核中的OsSRO1a和OsSRO1b蛋白相互作用.
- OsSRO1a 和 OsSRO1b 显著增强了 OsSWEET14 促进体的转录激活,通过 TAL 效应体 AvrXa7.
- 由AvrXa7诱导的OsSWEET14的表达在ossro1a突变体中被降低.
- 过度表达OsSRO1a导致疾病耐药性增加,与WRKY62和PBZ1.1等防御基因的高调表达相关.
- 发现OsSRO1a和OsSRO1b可以增强WRKY45的转录活性,这是WRKY62.2的关键调节者.
结论:
- 米的OsSRO1a和OsSRO1b蛋白质在通过细菌TAL效应器诱导的转录的积极调节中发挥着至关重要的作用.
- 这些OsSRO蛋白调节敏感性基因 (例如OsSWEET14) 和与防御相关的基因 (例如WRKY62,PBZ1) 的表达.
- OsSRO1a和OsSRO1b在植物病原体相互作用和宿主转录机制之间的相互作用中起着重要的调解作用.
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