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准宿主O链 glycan生物合成影响埃博拉病毒复制效率,并揭示了在埃博拉病毒糖蛋白上的差异性GalNAc-T受体位点偏好

Ieva Bagdonaite¹, Samir Abdurahman², Mattia Mirandola³

¹Department of Cellular and Molecular Medicine, Copenhagen Center for Glycomics, University of Copenhagen, Copenhagen, Denmark.

Journal of virology

|May 17, 2024

概括

埃博拉病毒糖蛋白 (EBOV GP) 的O-糖化被绘制成图,揭示了宿主GalNAc-T1的存在.

科学领域:

病毒学病毒学
葡萄糖生物学葡萄糖生物学
结构生物学结构生物学

背景情况:

埃博拉病毒糖蛋白 (EBOV GP) 广泛O-糖化,但其类域结构和宿主糖化影响尚不清楚.
与O相关的糖基化是由具有不同基质特异性的多GalNAc转移酶 (GalNAc-Ts) 启动的.

研究的目的:

在 EBOV GP.上绘制O-糖化位点的地图.
调查宿主O-糖化能力在EBOV复制中的作用.
探索调节EBOVGP糖化酶的潜在治疗点.

主要方法:

串联质谱测试用于在EBOV GP.上识别O-葡萄糖体.
野生类型和缺少GalNAc-T的HEK293细胞中的定量差异性O-糖蛋白组学.
在糖基工程细胞系中真正的EBOV传播.
模拟EBOV GP类域的分子动力学模拟.

主要成果:

在EBOV GP上鉴定出47个O-葡萄糖体,在不同的制剂中具有相似的模式.
发现了12个由GalNAc-Ts调节的O-糖化位点,主要是GalNAc-T1.1.
证明了宿主O链接甘氨酸的启动和延长对于病毒颗粒的产生和后代病毒标位至关重要.
绘制了O-甘氨酸的位置和结构,使分子动力学模拟成为可能.

结论:

主体O-糖化,特别是由GalNAc-T1启动,显著影响EBOVGP结构和EBOV复制.
针对GALNT1或C1GALT1C1提供了潜在的疫苗设计和干预策略,通过调节EBOV GP O-glycan密度来进行干预.
糖基工程细胞系是研究糖介导机制和指导免疫反应的宝贵工具.

关键词:

埃博拉病毒埃博拉病毒.这是GalNAc-TT.这是一种O-糖基化.葡萄糖转移酶的使用方法质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量类似于类动物的域名后翻译修改后的修改双重质量标签 (Tandem Mass Tag) 是一个双重质量标签.病毒型葡萄糖蛋白是一种病毒型葡萄糖蛋白.

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