相关实验视频
Updated: Jun 26, 2025

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Screening and Isolation of C-Glycoside-Cleaving Intestinal Bacteria
Published on: February 28, 2025
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来自Cayratia geniculata的糖化物组成部分
Dang Viet Cuong1,2,3, Tran Thi Hong Hanh1, Pham Thi Mai Huong1
1Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam.
Journal of Asian natural products research
|May 17, 2024
概括
研究人员从Cayratia geniculata中分离出六种甘氨酸衍生物,其中包括一种新的化合物 - - cayratioside. 凯拉提oside在细胞测试中对氧化产生的中度抑制作用.
科学领域:
- 植物化学 植物化学
- 自然产品 化学 化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 凯拉提亚 (Cayratia geniculata) 是一种具有潜在药用功能的植物物种.
- 糖化物是一种常见于具有多种生物活动的植物中的一类化合物.
- 氧化 (NO) 在炎症过程中起着至关重要的作用.
研究的目的:
- 从Cayratia geniculata中分离和描述化学成分.
- 评估隔离化合物对RAW264.7细胞中脂聚糖 (LPS) 诱导的氧化产生的抑制潜力.
主要方法:
- 使用各种色谱分离技术进行植物化学研究.
- 使用1D,2DNMR和HRESIMS对分离化合物的结构阐明.
- 在体外生物测定以测量LPS刺激的RAW264.7巨细胞中的氧化抑制.
主要成果:
- 成功分离了6种葡萄糖化物衍生物,其中包括一种新型的ent-labdane葡萄糖化物 (cayratioside, 1).
- 化合物1-6的结构通过全面的光谱分析得到证实.
- 凯拉提oside (1) 对LPS诱导的NO生产表现出中度的抑制活性,其IC50值为59.65±1.85μM.
结论:
- 凯拉提亚 (Cayratia geniculata) 是多种葡萄糖类衍生物的来源.
- 凯拉提oside是一种新的ent-labdane葡萄糖体,通过抑制氧化的产生,具有抗炎性质.
- 对凯拉酸和相关化合物的进一步研究可能会产生针对炎症状况的新型治疗剂.
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