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相关概念视频

Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

140
Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
140
Bioreactor Controls-II01:18

Bioreactor Controls-II

64
In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the...
64
Batch vs Continuous Culture01:14

Batch vs Continuous Culture

169
Fermentation is a foundational biotechnological process used to produce pharmaceuticals, biofuels, enzymes, and food additives. Among industrial strategies, batch and continuous fermentation are the two most widely applied. Although both rely on microbial conversion of substrates into desired products, they differ markedly in operation, productivity, and suitability for specific applications.Batch fermentation occurs in a closed system in which nutrient media and inoculum are added at the...
169
Fed-Batch Culture01:23

Fed-Batch Culture

137
Fed-batch culture is a widely used bioprocessing strategy combining aspects of batch culture with controlled substrate feeding to optimize cell growth and product formation. In this semi-closed system, nutrients are strategically added during fermentation, while the accumulated products and biomass remain within the bioreactor until the end of the operation. This controlled addition of substrates allows for better management of growth kinetics, nutrient limitation, and metabolite...
137
Scale-Up Processes01:14

Scale-Up Processes

89
The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
89

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相关实验视频

Updated: Apr 13, 2026

Scale-Up of Mammalian Cell Culture using a New Multilayered Flask
08:39

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在穿孔环瓶中进行扩散驱动的料批发发酵.

Clara Lüchtrath1, Felix Lamping1, Sven Hansen2

  • 1AVT-Biochemical Engineering, RWTH Aachen University, Forckenbeckstraße 51, 52074, Aachen, Germany.

Biotechnology letters
|May 17, 2024
PubMed
概括

这项研究引入了一种用于快速生物工艺开发的新型穿孔环瓶系统,与传统瓶相比,提高了3.5倍的氧气传输速率. 这一创新促进了生物技术中的高效选和流程优化.

关键词:
增材制造 增材制造是一种增材制造.联邦储备局批量批量氧气转移率是指氧气的转移速度.呼吸活动监测系统的呼吸活动监测系统.环形瓶瓶的使用方法

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Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture
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Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture

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相关实验视频

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科学领域:

  • 生物技术是生物技术.
  • 生物工艺工程 生物工艺工程
  • 化学工程是化学工程的重要组成部分.

背景情况:

  • 传统的Erlenmeyer水瓶在生物工艺开发过程中对监测和养有局限性.
  • 快速选和优化对于高效的生物技术进步至关重要.

研究的目的:

  • 在穿孔环瓶系统中引入基于膜的同时养和氧气传递率监测.
  • 为了实现快速的生物工艺开发和优化.

主要方法:

  • 开发了一个3D打印的适配器,用于在穿孔环瓶中监测氧气传递速率.
  • 使用大肠杆菌进行了批量和食批量实验.
  • 测试了不同的扩散速率和料溶液.

主要成果:

  • 穿孔环瓶在批量种植中实现了比Erlenmeyer瓶的3.5倍更高的氧气传输能力 (80 mmol L-1 h-1).
  • 成功的食批量实验证明了该系统在高葡萄糖度下的能力.
  • 通过监测氧气转移速率来确定氨的限制,并通过调整料成分来解决.

结论:

  • 基于膜的养,在线监测和穿孔环瓶的组合是生物工艺开发的一个有希望的工具.
  • 这种综合系统增强了生物技术中的查能力.
  • 该系统允许精确控制和优化生物过程.