黄尾金鱼 (Seriola lalandi) 中的胰岛素类生长因子结合蛋白-3 (igfbp-3) 和igfbp-5:不同营养状况和种群密度下的分子特征和表达水平
Jichang Zheng1, Wenjing Zhang1, Yongjiang Xu2
1State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China.
Fish physiology and biochemistry
|May 17, 2024
概括
研究人员在黄尾金鱼中确定了胰岛素类生长因子结合蛋白 (IGFBPs),揭示了它们在生长和发育中的作用. 这些IGFBPs还可以作为鱼类养殖中不利的培养条件的生物标志物.
科学领域:
- 水产养殖是水产养殖的一种方式.
- 鱼类生物学 鱼类生物学
- 分子生物学分子生物学
背景情况:
- 胰岛素样生长因子结合蛋白 (IGFBPs) 通过与IGF结合来调节生长.
- IGFBP-3和IGFBP-5是流通中的关键IGF载体.
研究的目的:
- 在黄尾金鱼 (Seriola lalandi) 中克隆和表征igfbp-3,igfbp-5a和igfbp-5b基因.
- 研究这些IGFBPs在鱼类生长,发育和应激反应中的表达模式和潜在作用.
主要方法:
- 从黄尾金鱼肝脏中克隆基因.
- 开放阅读框架 (ORF) 测序和氨基酸预测.
- 使用定量方法进行组织表达分析.
- 在胚胎和幼虫阶段进行表达概况.
- 在食物剥夺和高密度养殖下对基因表达的分析.
主要成果:
- 隔离的igfbp-3,igfbp-5a和igfbp-5b基因具有特定的ORF长度和编码的氨基酸计数.
- 证明了这三种基因的广泛组织表达,对igfbp-3和igfbp-5a/5b的不同模式.
- 在整个胚胎和幼虫发育过程中得到确认的表达.
- 在压力条件下 (食物剥夺,高密度) 观察到igfbp-3和igfbp-5a的显著上调,与生长相反相关.
结论:
- 在黄尾金鱼中确定了igfbp-3,igfbp-5a和igfbp-5b的分子特征.
- 这些IGFBP与黄尾金鱼的生长和发育有关.
- IGFBP-3和IGFBP-5a可以作为水产养殖不利条件的生物标志物.
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