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基于聚合诱导发射 (AIE) 的Cd2+聚体光探针的设计和应用

Jiang Zhou¹, Xuejiao Wang¹, Dajiang Wang²

¹Key Laboratory for Tibet Plateau Phytochemistry of Qinghai Province, College of Pharmacy, Qinghai Nationalities University, Xining, 810007, China.

Journal of molecular histology

|May 17, 2024

概括

一个新的多体光探针,TPE-HC,可以选择性地检测有毒的离子 (Cd2+),具有很高的灵敏度. 这种探测器可以在生物环境中快速检测和活细胞成像.

科学领域:

化学生物学化学生物学
环境科学环境科学
生物医学工程生物医学工程

背景情况:

(Cd2+) 是一种有毒的重金属,具有重大环境和人类健康风险.
准确检测Cd2+对于环境监测和健康评估至关重要.
现有的检测方法可能缺乏选择性,灵敏性或速度.

研究的目的:

开发一种新的光探针,用于选择性,敏感和快速检测Cd2+.
调查探测器在缓冲溶液和生物环境中的性能.
为了评估探测器适合于活细胞成像.

主要方法:

聚体光探针TPE-HC (TPE-His-Pro-Gly-Cys) 的合成和表征.
利用聚合诱导排放 (AIE) 效应用于Cd2+检测.
在Cd2+结合时测量光强度的变化.
确定检测极限并评估缓冲区和活细胞中的探针性能.

主要成果:

通过AIE效应,TPE-HC探针通过光强度的变化选择性检测到Cd2+ .
在缓冲溶液中实现了151 nM (R2 = 0.9933) 的低检测极限.
该探测器表现出高灵敏度,细胞透性,低生物毒性和活细胞成像中的有效性.

结论:

开发的TPE-HC探头为敏感和选择性Cd2+检测提供了一个有前途的工具.
它在生物环境中发挥作用并促进活细胞成像的能力为监测开辟了新的途径.
这项研究有助于开发用于检测有毒金属离子的先进生物传感器.

关键词:

聚合引发的排放量聚合引发的排放量在Cd2+上.细胞成像细胞成像聚酸光探针的光探测器

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