一个PACAP激活的分泌网络需要协调Ca2+流入和Ca2+调动
Xiaohuan Chen1, Nicole A Bell1, Breanna L Coffman1
1Department of Neurosciences, University of Toledo, Toledo, OH 43614.
Molecular biology of the cell
|May 17, 2024
概括
垂体腺酸激活聚 (PACAP) 通过维持高细胞内水平,触发了从上腺细胞中持续释放的压力激素. 这涉及从储存和流入中释放,由脂酶Cepsilon (PLCε) 调节.
科学领域:
- 神经内分泌学神经内分泌学
- 细胞信号传输 细胞信号传输
- 的恒温稳定 的恒温稳定.
背景情况:
- 克罗马芬细胞通过荷尔蒙分泌来调解压力反应.
- 在这个过程中,乙胆和PACAP是关键的神经递质.
- PACAP会诱导长时间的分泌,但其机制尚不清楚.
研究的目的:
- 为了阐明PACAP诱导的Chromaffin细胞持续分泌的信号机制.
- 确定在PACAP刺激期间调节细胞内动态的关键分子参与者.
主要方法:
- 通过成像,研究了 (Ca2+) 动力学在克罗马芬细胞中.
- 使用L型通道和耗尽内部储量的药理抑制剂.
- 评估了因诺三酸盐受体 (IP3Rs) 和脂酶Cepsilon (PLCε) 在PACAP信号传递中的作用.
主要成果:
- PACAP诱导持续的细胞溶液Ca2+增加,这取决于Ca2+的流入和内部储存释放.
- 通过IP3Rs,PACAP引发的Ca2+从内 плазма网膜 (ER) 释放出来,将动员与流入合起来.
- 两种Ca2+流入和动员途径都需要脂酶Cepsilon (PLCε) 活性.
结论:
- 通过促进持续的细胞内Ca2+升高的网络,PACAP通过一个网络调节持续的克罗马芬细胞分泌.
- 这些发现揭示了一种新的信号通路,涉及PLCε,IP3R和通道在释放压力激素中的作用.
- 了解这种机制,可以了解神经内分泌应激反应.
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