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达卡糖尿病足患者的细菌学分析和抗生素耐药性

Poulomi Baral¹, Nafisa Afnan¹, Maftuha Ahmad Zahra¹

¹Microbiology Program, Department of Mathematics and Natural Sciences, BRAC University, Dhaka, Bangladesh.

|May 17, 2024

概括

糖尿病足含有各种细菌,其中Klebsiella pneumoniae是最常见的. 抗多种药物的大肠杆菌流行,而グラム阴性细菌对piperacillin-tazobactam具有很高的敏感性.

科学领域:

微生物学微生物学
传染性疾病传染性疾病
糖尿病学糖尿病学

背景情况:

糖尿病足 (DFU) 是糖尿病的一个重要并发症,经常导致严重的感染.
了解DFU中的微生物景观和抗生素耐药性模式对于有效治疗至关重要.
2型糖尿病和胰岛素使用在DFU患者中很普遍,影响了感染特征.

研究的目的:

为了隔离和识别来自糖尿病足的细菌.
为了确定分离的细菌的抗生素耐药性概况.
为了将细菌发现与的严重程度相关联 (瓦格纳尺度).

主要方法:

从75名患有足部的糖尿病患者收集了样.
使用选择性介质,生物化学测试和聚合酶链反应 (PCR) 进行细菌分离和鉴定.
对141个细菌分离物进行了抗生素敏感性测试.

主要成果:

总共获得了141种细菌分离物,其中格拉姆阴性细菌 (82.97%) 主导.
最常被隔离的物种是Klebsiella pneumoniae,其次是Proteus spp.,大肠杆菌, Pseudomonas aeruginosa和金黄色葡萄球菌.
大肠杆菌表现出最高的多药耐药性 (81.81%). 格拉姆阴性细菌在很大程度上对安培素有耐药性,但对piperacillin-tazobactam敏感.
黄金葡萄球菌对万科米辛和诺夫洛克萨辛表现出敏感性.

结论:

DFU经常是多微生物的,其中负细菌是主要的病原体.
多种药物耐药性的高率,特别是大肠杆菌,需要仔细选择抗生素.
皮佩拉西林-塔扎巴克坦对格拉姆阴性分离物具有广泛的疗效,而万科米辛和诺夫洛克萨辛对金黄色葡萄球菌 (Staphylococcus aureus) 有效.

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