在对环境压力的抵抗期间,Enterococcus faecalis生物膜中蛋白质表达的差异
Wei Jiang1,1, Youmeng Zhang2,1, Jie Yan1,1
1Department of Stomatology, Shanghai Hudong Hospital, Shanghai, China.
概括
这项研究使用蛋白质学研究了Enterococcus faecalis生物膜对和酸盐 (NaOCl) 的反应. 研究人员发现了蛋白质表达的显著变化,特别是ATP结合盒 (ABC) 载体,这对于在压力下生存至关重要.
科学领域:
- 微生物学 微生物学
- 蛋白质组学是指蛋白质组学.
- 牙周内科医院 牙周内科医院 牙周内科医院
背景情况:
- 肠球菌 (Enterococcus faecalis) 生物膜在根管壁上普遍存在,它们对消毒剂有抗性.
- 这种病原体的持久性与其承受高性和抗菌剂等恶劣条件的能力有关.
- 以前的研究缺乏全面的蛋白质组学来理解Enterococcus faecalis对和NaOCl的应激反应.
研究的目的:
- 在极端环境压力下研究Enterococcus faecalis生物膜的蛋白质表达模式.
- 为了确定参与Enterococcus faecalis对高pH和酸盐的应激反应的蛋白质.
主要方法:
- 在实验室中建立了一个Enterococcus faecalis生物膜模型.
- 基于液体染色学和质谱学 (LC-MS/MS) 的无标签定量蛋白质组学被用来比较pH10和5%NaOCl下的蛋白质表达.
- 量化PCR (qPCR) 和并行反应监测 (PRM) 用于验证.
主要成果:
- 在暴露在高性和NaOCl条件下的Enterococcus faecalis生物膜中确定了大量的蛋白质.
- 与ATP结合盒 (ABC) 载体相关的蛋白质得到了显著的丰富.
- 在高性压力下确定了15个高度表达的ABC载体,其中7个显示超过1.5倍的增加.
结论:
- 这项研究揭示了当抗拒环境压力时,Enterococcus faecalis生物膜内的蛋白质表达的实质性改变.
- 这些发现增强了对这种病原体中差异性蛋白质表达和环境应激抵抗之间的关系的理解.
相关概念视频
Biofilms
2.1K
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
2.1K
Gene Regulation in Microbial Communities: Quorum Sensing
954
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
954
Stringent Response in E. coli
528
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
528
Development of Antibiotic Resistance
2.0K
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
2.0K
Mechanism of Antibiotic Resistance in MRSA
219
Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and...
219
Clinical Significance of Antibiotic Resistance
90
Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...
90


