马特林通过触发ROS/AMPK/mTOR轴和多发性骨髓瘤中亡来诱导自细胞死亡
Xue Li1, Jifan Zhou2, Yixin Ling1
1Department of Hematology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China; Institute of Hematology, Wenzhou Medical University, Wenzhou, Zhejiang, China; Wenzhou Key Laboratory of Hematology, Wenzhou, Zhejiang, China.
概括
马特林,来自Sophora flavescens,触发了多发性骨髓瘤 (MM) 细胞的亡和自. 将马林与拉巴胺素结合起来可以增强抗MM作用,这表明是一种有前途的治疗策略.
科学领域:
- 在瘤学瘤学.
- 药理学 药理学是指药理学的学科.
- 细胞生物学 细胞生物学
背景情况:
- 多发性骨髓瘤 (MM) 仍然是一个重大挑战,因为治疗耐药性和复发.
- 马特林是一种来自Sophora flavescens的化合物,具有抗瘤潜力.
- 自在马特林抗MM作用中的作用尚不清楚.
研究的目的:
- 调查自在母体诱导的抗多发性骨髓瘤效应中的作用.
- 阐明母蛋白在MM细胞中的作用的潜在分子机制.
- 为了评估结合马特林与拉帕米辛用于MM治疗的协同潜力.
主要方法:
- 在体外研究中,使用用母体蛋白,ATG5siRNA,spautin-1和拉巴胺素治疗的MM细胞系.
- 对亡,细胞循环停止,自标志物,活性氧物种 (ROS) 和AMPK/mTOR信号的分析.
- 使用异种移植小鼠模型进行体内研究,以评估matrine和rapamycin的联合疗效.
主要成果:
- 孕妇诱导的亡,细胞循环停止和MM细胞的自.
- 自的抑制部分逆转了马林的抗MM作用,而拉巴素则增强了它们.
- 马特林增加了ROS水平,并激活了AMPK/mTOR通路.
- 合并的马林和拉巴胺在体内显示出协同作用的抗瘤活性.
结论:
- 在多发性骨髓瘤细胞中,Matrine通过ROS/AMPK/mTOR途径诱导自.
- 自在调解母体的抗MM作用方面发挥着至关重要的作用.
- 与马林和拉帕米辛的联合治疗为治疗多发性骨髓瘤提供了一个有希望的策略.
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