一种基于表面活性剂介导的表面滴滴蒸发的生物传感器方法,用于检测阿弗拉托克辛B1
Supan Cheng1, Fangchao Yin2, Zhongxing Wang2
1Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China; School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China; Qingdao Academy of Chinese Medical Sciences, Shandong University of Traditional Chinese Medicine, Qingdao 266112, China.
Food chemistry
|May 17, 2024
概括
一个新的生物传感器使用表面活性剂滴滴蒸发检测阿弗拉托克辛B1 (AFB1). 这种简单,无标签的方法可以达到较低的检测极限,从而提高食品安全.
科学领域:
- 分析化学 分析化学
- 生物传感技术的技术
- 食品安全 食品安全
背景情况:
- 非洲毒素B1 (AFB1) 是一种普遍存在的真菌毒素,对全球食品安全构成重大风险.
- 目前的检测方法可能是复杂和昂贵的.
- 需要简单,灵敏和负担得起的AFB1检测技术.
研究的目的:
- 开发一个创新的传感平台来检测AFB1.1.
- 为了利用表面活性剂介导的表面滴滴蒸发用于AFB1量化.
- 为AFB1分析建立一个简单,无标签,且具有成本效益的生物传感器.
主要方法:
- 基于在疏水表面蒸发表面活性剂溶液 (CTAB) 的生物传感器的开发.
- 由于CTAB/亚胺复合体的形成和AFB1-亚胺特异性结合,干燥图案大小的变化的观察.
- 利用干燥图案大小的变化作为AFB1存在和度的指标.
主要成果:
- 形成一个CTAB/体复合体减少了干燥的图案大小.
- AFB1与其体的特定结合增加了干燥模式的大小.
- 在AFB1检测中达到0.77 pg/mL的敏感检测极限.
结论:
- 表面活性剂介导的表面滴水蒸发生物传感器是AFB1检测的可行方法.
- 这种无标签,简单,廉价的方法对食品安全应用有很大的前景.
- 该方法为菌毒素监测提供了一个方便和有效的策略.
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