工程酵母可同时利用甲醇或甲酸盐与CO2固定相结合
Yuanke Guo1, Rui Zhang1, Jing Wang1
1State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, Jiangsu, China.
Metabolic engineering
|May 17, 2024
概括
工程酵母Pichia pastoris和Saccharomyces cerevisiae现在可以利用二氧化碳与甲醇或酸盐,这要归功于合成MFORG途径. 这一突破使得C1化合物的同化能够有效地用于潜在的化学生产.
科学领域:
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
- 微生物生物技术 微生物生物技术
背景情况:
- 能够利用一碳 (C1) 化合物如二氧化碳,甲醇和酸盐的微生物对于可持续的化学生产具有重要意义.
- 在像Pichia pastoris和Saccharomyces cerevisiae这样的模型酵母中开发C1同化合成途径仍然是一个关键的挑战.
研究的目的:
- 设计Pichia pastoris和Saccharomyces cerevisiae用于合成甲基和格式,使甲醇或格式与二氧化碳固定一起使用.
- 在这些酵母宿主中建立和验证合成C1化合物同化通路 (MFORG通路).
主要方法:
- 组装MFORG通路,包括甲醇-甲酸盐氧化模块和还原型甘氨酸通路.
- P. pastoris的基因工程,包括阻断本地甲醇同化和模块化工程MFORG通路基因.
- 在S. cerevisiae中引入MFORG通路,并通过13C-标志物分析确认C1化合物的利用.
主要成果:
- 成功设计了P. pastoris以利用甲醇和甲酸盐.
- 将MFORG路径转移到S. cerevisiae中,建立了具有显著消费率的合成甲基和格式.
- 在两种工程酵母菌株中都证明了二氧化碳与甲醇或甲酸盐的共同同化.
- 在工程酵母中使用甲醇和二氧化碳的协同同化生成的5-aminolevulinic酸和乳酸.
结论:
- 在P. pastoris和S. cerevisiae中,MFORG通路使合成甲基和格式成为可能.
- 工程酵母可以高效地与二氧化碳固定共同利用C1化合物 (甲醇/甲酸盐).
- 这项工作突出了MFORG途径在开发C1基化学品生产中多样化的微生物宿主方面的潜力.
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