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第三代PacBio测序用于探索肠道细菌和结直肠癌中的性别

Sheng Yu¹, Jian Chu¹, Yinhang Wu¹

¹Huzhou Central Hospital, Affiliated Central Hospital Huzhou University, Zhejiang Province, People's Republic of China; Fifth School of Clinical Medicine of Zhejiang Chinese Medical University (Huzhou Central Hospital), Zhejiang Province, People's Republic of China; Key Laboratory of Multiomics Research and Clinical Transformation of Digestive Cancer of Huzhou, Zhejiang Province, People's Republic of China.

Microbial pathogenesis

|May 17, 2024

概括

在结直肠癌 (CRC) 患者中,肠道细菌的组成在性别之间有显著差异. 识别性别特定的细菌,如男性的Peptostreptococcus anaerobius和女性的Blautia stercoris,对于CRC诊断和预防至关重要.

科学领域:

微生物组研究的研究.
胃肠病学胃肠病学
在瘤学瘤学.

背景情况:

肠道细菌在结直肠癌 (CRC) 的发展中发挥着重要作用.
新出现的证据表明,肠道微生物组成的性别特异性差异.

研究的目的:

研究肠道细菌与被诊断患有结直肠癌的患者的性别差异之间的关系.
为了确定CRC的潜在的性别特异性微生物生物标志物.

主要方法:

从212名结肠直肠癌患者和212名健康志愿者收集了便样本.
第三代PacBio测序用于分析肠道微生物组成.
线性差异分析效果大小 (LEfSe) 和皮尔森相关性用于识别差异性细菌及其关联.

主要成果:

在男性和女性CRC患者中,Escherichia flexneri和Phocaeicola vulgatus都是普遍存在的.
观察到明显的细菌丰富:男性的细菌,Verrucomicrobia和Akkermansiaceae;女性的细菌,Phocaeicola和Tissierellales.
佩普托斯特雷普托科克斯 (Peptostreptococcus anaerobius) (AUC=0.951) 和布劳蒂亚斯特科里斯 (Blautia stercoris) (AUC=0.966) 成为关键的性别特异性CRC相关细菌.

结论:

肠道细菌的组成在结直肠癌患者的性别之间表现出显著的差异.
在使用肠道细菌用于CRC诊断和预防策略时,性别应该是关键考虑因素.

关键词:

结肠直肠癌是一种癌症.埃舍里希亚柔性菌 (Escherichia flexneri) 是一种的植物.性别性别性别性别肠道细菌是什么肠道细菌是什么风险预测风险预测

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