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对质细胞特异性SNP和nSCoT标记物的比较分析和果植物群体结构研究

Yinling Ding^1,2,3,4, Yu Wang^1,2,3,4,5, Zhe Chen^1,2,3,4

¹School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi Province, China.

|May 17, 2024

概括

基维果的遗传多样性使用质细胞特异的单核酸多态 (SNP) 和核简单序列重复 (SCoT) 标记物进行了分析. 叶绿体DNA (cpDNA) 标记物,特别是单元型,在揭示Actinidia属内的种群结构和进化关系方面被证明是最有效的.

科学领域:

遗传学是一个遗传学.
植物科学植物科学
进化生物学进化生物学

背景情况:

基维果 (Actinidia) 由于自然杂交和多化,具有显著的遗传多样性.
中国是关键Actinidia物种的来源和化中心,包括A. chinensis var. 华人,A. chinensis var. 华人的一种. 美味的,A.Arguta,和A.多妻的.
有限的研究已经探索了基因基础和DNA标记器在果繁殖中的应用.

研究的目的:

为了评估基的种群结构和遗传变异,使用结合的质细胞特异性SNP和核SCoT标记物.
为了揭示Actinidia属内的进化关系和哈普洛型多样性.
确定有效的分子标记物,用于基维果的遗传研究和育种.

主要方法:

选择了一个质细胞DNA (cpDNA) 标记对和20个核SCoT (nSCoT) 标记.
通过使用这些标记,分析了来自四种物种/品种的55个基维果样本.
用贝叶斯分析,UPGMA和PCA来评估人口结构.
从205个可变位点中鉴定出15个质体特异性的DNA杂型.

主要成果:

质细胞特异性SNP标记物比nSCoT标记物解释了更多的遗传变异,cpDNA单元型是最有效的.
观察到高多态性 (Hd = 0.78) 与15个cpDNA单元型,多样性分布在群体之间和群体内.
确定了一种由A. chinensis var.共享的占主导地位的古老单元型 (H1). 中华和A. chinensis var. 这两种. 美味的,而A.Arguta和A.多夫妇显示出不同的单体类型.

结论:

叶绿体DNA标记物,特别是单质类型,对于分析果的遗传变异和种群结构是优越的.
在A. chinensis var.之间显而易见的基因流动. 中华和A. chinensis var. 这两种. 美味的,由共享的单元型表示.
A. arguta和A. polygama拥有来自A. chinensis的独特单体,这表明了不同的进化轨迹以及选Actinidia属核心标志物的重要性.

关键词:

活动性活动性活动性叶绿体特异性的DNA单体样型多样性塑固体特异性的SNP 人口结构人口结构这就是为什么nSCoTT.

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