相关实验视频

Updated: Jun 26, 2025

A Gnotobiotic System for Studying Microbiome Assembly in the Phyllosphere and in Vegetable Fermentation

A Gnotobiotic System for Studying Microbiome Assembly in the Phyllosphere and in Vegetable Fermentation

Published on: June 3, 2020

来自水果和蔬菜废物厂的发酵:通过抑制定数感应来降低土壤中人类细菌病原体的风险

Lin Zhu¹, Jingpeng Li¹, Jian Yang¹

¹Science and Technology Cooperation Platform for Low-Carbon Recycling of Waste and Green Development, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, Zhejiang, China; Provincial Key Laboratory of Solid Waste Treatment and Recycling, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China.

Environment international

|May 18, 2024

概括

来自水果和蔬菜废物的发酵 (FFVW) 有效地减少有害的土壤微生物,包括携带抗生素耐药性基因 (ARG) 和毒性因子基因 (VFG) 的人类细菌病原体 (HBPs). 这种环保方法的目标是为改善土壤和人类健康的定数感应 (QS) 途径.

科学领域:

环境微生物学环境微生物学
土壤科学土壤科学
公共卫生公共卫生

背景情况:

来自水果和蔬菜废物的发酵 (FFVW) 是一种已知的土壤修改剂,可以减少抗生素耐药性基因 (ARG).
FFVW对土壤中人类细菌病原体 (HBPs) 和毒性因子基因 (VFGs) 的影响尚不清楚.
型肝炎和型肝炎对人类健康构成重大风险.

研究的目的:

调查FFVW在缓解HBPs和VFGs污染土壤中的有效性.
阐明FFVW影响这些微生物污染物的机制.
评估FFVW作为对土壤和人类健康的环保解决方案的潜力.

主要方法:

用FFVW处理的土壤样本的基因组学分析.
对HBPs,VFGs和ARGs的量化.
纯种植实验以验证发现.
检验决数感应 (QS) 相关基因和信号的分析.

主要成果:

FFVW显著降低了高风险HBPs的丰富性,例如Klebsiella肺炎,Mycobacterium结核病和Streptococcus肺炎.
土壤VFG的丰度下降,QS信号和相关基因 (例如,rpaI,luxS,rpfC,fusk) 的显着减少.
纯培养实验证实了FFVW治疗的关键病原体中QS,VFG,ARG和移动遗传元件 (MGE) 基因表达的下调.

结论:

FFVW是一种有效的,环保的方法,用于控制携带VFG和ARG的土壤传播HBPs.
这种机制涉及到抑制定数感应途径.
这种方法通过改善土壤和人类健康来支持"一个健康"框架.

关键词:

发酵是发酵中的一种.人类细菌病原体的病原体.微生物污染的发生议员数量检测是指对议员数量进行检测.病毒性因子基因病毒性因子基因.

更多相关视频

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases

Published on: January 1, 2016

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

Published on: May 27, 2022

相关概念视频

Fates of Pyruvate

Fates of Pyruvate

Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...