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在对人类冠状病毒OC43感染的反应中,BHK21细胞系的转录组动态

Mianhuan Li¹, Yang Yang², Pusen Wang²

¹School of Medicine, Southern University of Science and Technology, Shenzhen 518055, People's Republic of China; Shenzhen Third People's Hospital, National Clinical Research Centre for Infectious Disease, The Second Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518112, People's Republic of China.

Microbiological research

|May 18, 2024

概括

人类冠状病毒OC43 (HCoV-OC43) 感染会影响Wnt/β-catenin通路,在早期和晚期不同影响病毒复制. 这一途径是潜在的抗病毒点.

科学领域:

病毒学病毒学
分子生物学分子生物学
细胞信号传输细胞信号传输

背景情况:

病毒感染涉及复杂的转录调节.
了解病毒复制和病变需要识别新的病毒转录.
人类冠状病毒OC43 (HCoV-OC43) 是一个重要的呼吸道病原体.

研究的目的:

为了研究在HCoV-OC43感染期间宿主细胞RNA的动态变化.
阐明Wnt/β-catenin信号通路在HCoV-OC43感染中的作用.
探索潜在的针对Wnt/β-catenin通路的抗病毒策略.

主要方法:

利用醇 (SH) 相关化进行RNA代谢测序 (SLAM-seq) 进行时间解析的RNA分析.
应用代谢RNA标记,以区分BHK21细胞中的稳定状态和新合成的RNA.
研究了Wnt通路抑制和雷梅西维尔治疗对病毒复制和RNA水平的影响.

主要成果:

HCoV-OC43感染显著丰富了BHK21细胞中与Wnt/β-catenin信号通路相关的新合成的转录.
抑制Wnt途径对病毒复制产生了差异性影响:促进早期复制,抑制晚期复制.
发现雷梅西维尔可以抑制HCoV-OC43诱导的Wnt/β-catenin通路的上调.
尽管有细胞病变效应,亡抑制并没有影响细胞内HCoV-OC43复制.

结论:

在HCoV-OC43感染的不同阶段,Wnt/β-catenin通路发挥着多方面的作用.
针对Wnt/β-catenin通路为HCoV-OC43感染提供了一个潜在的治疗策略.
使用SLAM-seq的时间解析RNA监测是研究病毒感染机制的宝贵方法.

关键词:

在HCoV-OC43中.介绍介绍介绍介绍雷姆迪西维尔 (Remdesivir) 是一种药物.这就是SLAM-seqq.病毒感染是一种病毒感染.没有路径的路径.

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