IKVAV功能化定向的PCL/FeO支架用于磁性调节DRG生长行为.
Yaqiong Liu1, Hongxia Gao2, Yuqing Shang2
1Key Laboratory of Neuroregeneration, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong 226001, PR China; Guangdong Provincial Key Laboratory of Advanced Biomaterials, Southern University of Science and Technology, Shenzhen 518055, PR China.
Colloids and surfaces. B, Biointerfaces
|May 18, 2024
概括
具有IKVAV酸功能的磁性PCL/Fe3O4支架促进了外围神经的再生. 这些由磁场引导的生物相容支架增强神经生长因子分泌和轴突延伸,用于神经组织工程应用.
科学领域:
- 生物材料科学 生物材料科学
- 神经组织工程 神经组织工程
- 再生医学是一种再生医学.
背景情况:
- 外围神经损伤对功能恢复构成重大挑战.
- 有效的神经再生需要指导线索用于轴突延伸和细胞生长.
- 现有的治疗方法往往无法完全恢复神经功能.
研究的目的:
- 开发和评估方向对齐的磁性聚烯/三铁四氧化物 (PCL/Fe3O4) 纤维支架.
- 用IKVAV来功能化这些支架,以促进背部根 (DRG) 的生长和轴突延伸.
- 研究磁场和IKVAV对外围神经再生的协同效应.
主要方法:
- 使用电制造对齐的PCL/Fe3O4复合材料支架.
- 使用IKVAV的脚手架的功能化.
- 对脚手架的生物相容性和细胞毒性进行评估.
- 在体外和体内对DRG和轴突生长的评估.
- 使用ELISA对神经生长因子 (NGF) 分泌的分析.
- 在静态磁场 (SMF) 刺激下进行基因表达分析 (Cntn2,PCNA,Sox10,Isca1).
主要成果:
- 成功准备了面向的,对齐的,具有良好的机械性能和磁性响应的磁性PCL/Fe3O4支架.
- 脚手架表现出极好的生物相容性和低细胞毒性.
- 在IKVAV功能化的支架上,指导方向轴突延伸.
- 外部磁场应用和IKVAV接种显著促进了DRG和轴突生长.
- 支架在SMF下增加了NGF分泌,促进了轴突生长.
- 在SMF下观察到与粘附,增殖和磁性受体功能相关的基因显著上调.
结论:
- 具有IKVAV功能的PCL/Fe3O4复合面向支架对外围神经再生具有前景.
- 磁导和IKVAV的组合可以增强神经的修复.
- 这些支架显示了神经组织工程应用的潜力,改善神经损伤后的功能恢复.
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