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Gan Wu¹, Zhaoyang Ding², Xilin Dou¹

¹College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

|May 18, 2024

概括

开发了一种新的aptamer光传感器,用于检测组胺. 这种传感器可以快速准确地分析食品中的组胺,确保食品安全和质量控制.

科学领域:

分析化学分析化学
生物技术是生物技术.
食品科学食品科学食品科学

背景情况:

希斯胺是生物氨基毒素中毒的重要原因.
严格控制食品中的组胺水平对于公众健康至关重要.
现有的检测方法可能缺乏实时监控的速度或准确性.

研究的目的:

开发一种新型的aptamer光传感器,用于敏感和特定的组胺检测.
为提高准确性创建一个比度检测平台.
在真实食品样本和生物成像中评估传感器的性能.

主要方法:

制备PF8BT - 聚合物点 (PF8BT-Pdots) 使用Poly[-9,9-di-n-octylfluorenyl-2,7-diyl) -alt-(benzo [2,1,3] thiadia-zol-4,8-diyl) ] (PF8BT) 和烯马来酸无水化共聚物 (PSMA).
结合PF8BT-Pdots与氨酸3-胺酸 (Cy3) 和基因组胺特异性吸收体,用于基于光共振能量转移 (FRET) 的比度检测.
用刺水产养殖水和金枪鱼样本测试传感器,并对HeLa细胞进行细胞成像.

主要成果:

光传感器实现了线性检测范围为3-21μmol/L的组胺.
获得了0.38μmol/L的低检测极限 (LOD).
在经过处理的水产养殖水和金枪鱼样本中成功检测出组胺.
在HeLa细胞成像中观察到出色的生物相容性和光成像能力.

结论:

开发的aptamer光传感器为组织胺检测提供了一个新的,快速和准确的平台.
这种传感器显示出在食品安全和质量控制领域的应用潜力很大,尤其是水产产品.
这项研究强调了基于aptamer的光传感器在敏感生物分子检测中的实用性.

关键词:

亚普特美尔 (Aptamer) 是一种药物.细胞成像细胞成像食品安全食品安全基因组胺是一种基因组胺聚合物点是指聚合物点.

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