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在二硫化碳应力下对化污泥进行多omics分析:化性能和分子机制

Qian Liu¹, Jie Chen¹, Qi Zhou²

¹Research Center for Eco-environmental Engineering, Dongguan University of Technology, Dongguan, 523808, China.

|May 18, 2024

概括

二硫化碳 (CS2) 通过压力化污泥对废水处理产生影响. 这项研究揭示了CS2的存在.

科学领域:

环境科学环境科学
生物技术是生物技术.

背景情况:

二硫化碳 (CS2) 是一种具有细胞毒性特性的关键工业化学物质.
废水中的剩余CS2可能会对处理厂中化污泥的性能产生负面影响.
对CS2对化污泥的影响的研究是有限的.

研究的目的:

调查化污泥在短期和长期CS2压力下的性能.
分析CS2对微生物细胞的毒性影响.
阐明微生物社区结构的继承和代谢产品的变化.

主要方法:

将化污泥暴露在不同度的CS2中.
测量氧化应激标志物 (ROS,SOD,催化酶).
酶活性测定 (酸盐氧化降解酶).
细胞外聚合物质 (EPS) 的分析.
微生物社区结构分析 (16S rRNA测序).
代谢分析.代谢分析.

主要成果:

低CS2度诱导和细胞修复氧化应激.
100-200 mg/L CS2 抑制了抗氧化酶和酸盐氧化降解酶.
长时间的压力增加了可溶性EPS和CS2与硫酸盐的同化.
微生物群落从罗达诺巴克特转移到西诺马纳斯.
CS2影响了关键的代谢途径,包括泛多酸和CoA生物合成.

结论:

CS2对化污泥具有剂量依赖的毒性,影响微生物群落和代谢功能.
了解这些机制对于减轻废水处理中的CS2抑制至关重要.
未来的策略可能包括添加金属离子或选择/修改降解CS2的细菌.

关键词:

碳二硫化碳的使用情况.代谢活动代谢活动.微生物社区的微生物社区.在化过程中,化氧化应激是一种氧化应激.

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